Protein Info for BBR_RS13995 in Bifidobacterium breve UCC2003

Annotation: tyrosine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 TIGR00234: tyrosine--tRNA ligase" amino acids 14 to 436 (423 residues), 376.3 bits, see alignment E=1e-116 PF00579: tRNA-synt_1b" amino acids 40 to 334 (295 residues), 269.9 bits, see alignment E=2.8e-84 PF01479: S4" amino acids 381 to 414 (34 residues), 26.6 bits, see alignment 4.1e-10

Best Hits

Swiss-Prot: 100% identical to SYY_BIFLD: Tyrosine--tRNA ligase (tyrS) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K01866, tyrosyl-tRNA synthetase [EC: 6.1.1.1] (inferred from 99% identity to blo:BL1051)

Predicted SEED Role

"Tyrosyl-tRNA synthetase (EC 6.1.1.1)" (EC 6.1.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>BBR_RS13995 tyrosine--tRNA ligase (Bifidobacterium breve UCC2003)
MAHVTDFKEAGFNTLLEELEWRGLISQSTDRDRLAEALNGEPITYYCGFDPTAASLHIGN
LVQLINMRHLQLAGHHPIALVGGATGLIGDPRQSGERTLNPKDVVAGWADRLKNQIGGIL
ETEGANPVRFVSNYDWTASMTVIDFLRDVGKNFRLGTMLAKDTVARRLNSEEGISFTEFS
YQVLQGNDFLHLFDEYHCTLELGGSDQWGNLTSGLDLIHKVRGVDVNVFTSPIITDASGK
KFGKSEGNAVWLDATMLSPYKFYQFWINRPDVEMESLLKAFTFLPKAEIERLVEESKTNP
GKREAQKTLAWEVTSFVHGEAATQAAIDASGALFGRGGNLEDIDEETLESVLDGFKVVDE
NGEHVFPVSKPGDRVIDAAQAAGLFKSASEARRAIKSGGVYLNNNRIEDEEQVLAEADFL
AGRFALIRRGKKALGAVENR