Protein Info for BBR_RS13875 in Bifidobacterium breve UCC2003

Annotation: phenylalanine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 869 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 6 to 868 (863 residues), 591.1 bits, see alignment E=1.9e-181 PF01588: tRNA_bind" amino acids 47 to 149 (103 residues), 28.8 bits, see alignment E=2.6e-10 PF03483: B3_4" amino acids 251 to 428 (178 residues), 221.3 bits, see alignment E=1.6e-69 PF03484: B5" amino acids 448 to 514 (67 residues), 63.7 bits, see alignment 3.7e-21 PF17759: tRNA_synthFbeta" amino acids 517 to 758 (242 residues), 173.3 bits, see alignment E=1.3e-54 PF03147: FDX-ACB" amino acids 776 to 868 (93 residues), 92.8 bits, see alignment E=3.6e-30

Best Hits

Swiss-Prot: 94% identical to SYFB_BIFLO: Phenylalanine--tRNA ligase beta subunit (pheT) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 94% identity to bll:BLJ_0634)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (869 amino acids)

>BBR_RS13875 phenylalanine--tRNA ligase subunit beta (Bifidobacterium breve UCC2003)
MPMIDIDWLKDHVEVPEGLTYEQLAKDLVKVGLEEEEIHSSQVTGPIVVGYVVDATPEPQ
KNGKVINWCHVDCGDEWNEADENGNKVPRGIICGAPNMKAGEKVVVTLPGAVLPGDFKIE
PRKTYGHISNGMCASERELGLGDNHNGIILLREYGFSEAEYEALKPGQDAMHLLHLDQPL
LEINITPDRGYTLSYRGVAREYHHSTGAVYTDPAIALNEKAPEPADYQPGTPVDIDIEID
DNNPIHGVPGCDRYYARVVKGFDPNAHTPSWMRRRLIRAGMRSISLAVDVTNYVMLDLGQ
PMHAYDLDKLEGPIVVRRANEGEKLTTLDGKEHDLSTEDLLITDSPNGERGSRILGLAGV
MGGLYGEVTADTKNILLEAAHFDQVTIARSARRHKIPSEASRRFERGVDTELQPAAAQMA
AELMAKYGNGEPSEHPNDVDNTPRPKVIHFKASEVARVAGLDVDINRISDILTDIGCVVA
GGGNGEFSITAPSWRPDLGEPCDLVEEIARLVGYDQIPVTVPPAPVEGLVGLTPDQTRRR
RVADELAEYGMVEALSYPFVGDDDYRAFGFDPEATKKVSVEIANPLYGDRPYLRRDILPT
LATTVQRNIRRGIENVSLYELGHVYLWDPNAPAIPSLPGGVRPTDEQLAALDAGLPDQPD
HVAGILTGLAEDDGWLGGKRPVDWSDAVEAVHRLADRIGAKIVLDQPAAEDVPAQWHPGR
AARVMVGDVFTGWVGELHPRVNEALGFPAHSAAFELNLTALFGTLTGKPVQAKPISSFPP
VKQDLAFTVDASVTADQLEAVIREAAGANLESIELFDVFTGEQVGEGKKSLAYAVVFRSP
DKTLSADDNDAIRQAIVAKAADLGAQLRA