Protein Info for BBR_RS13835 in Bifidobacterium breve UCC2003
Annotation: FAD-dependent oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 86% identity to blf:BLIF_0561)Predicted SEED Role
"Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4)" in subsystem TCA Cycle (EC 1.8.1.4)
MetaCyc Pathways
- glycine biosynthesis II (2/3 steps found)
- glycine cleavage (2/3 steps found)
- 2-oxoglutarate decarboxylation to succinyl-CoA (1/3 steps found)
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (1/3 steps found)
- pyruvate decarboxylation to acetyl CoA I (1/3 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (12/22 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycine, serine and threonine metabolism
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.4
Use Curated BLAST to search for 1.8.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-positive bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (497 amino acids)
>BBR_RS13835 FAD-dependent oxidoreductase (Bifidobacterium breve UCC2003) MNEQFDLAIIGAGPGGYSTALRAAELGMSVALIERDATVGGTCLNRGCIPSKALITATRT IDTVHRAAELGVNASVNGIDFGTLRDYRLRVVDTMVKGLAGLLAHRNITVLRADASFNAE ETDAADNARHIVHLAPSPGQTEILTYHKADVPEPTDASLDLAAGNVVIATGAKPRPLPGN PFAGALIDSTQALEVNEFPSSAVIIGAGAIALEFASMWNAAGSQVTLLIRKNRVLSTWDR RAGTTLTRELKRRGVNVITHTIVTHVDTGANLGATVHYTREGQDSEQSVWGEIALAAIGR TPVADPSWGVALTESGHVATDAFGRTNKSGIWAVGDVTPGHALAHRAFEQGIVIAETIAG LNPKPVDENTVPQIVFSSPEAASVGLTIEQAQARENLLEVKETIYPMLANARMLMSGTAG SLTIVSGCDAADPDTPRVLGVHMVSPVASDIISEAEQLVGNRLPLADAARLIHPHPTFSE TLGEALLKADDRPLHTR