Protein Info for BBR_RS13635 in Bifidobacterium breve UCC2003

Annotation: carbamoyl-phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1096 (1095 residues), 1384.1 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 222.6 bits, E=1.7e-69
  • amino acids 677 to 885 (209 residues), 124.1 bits, E=2.3e-39
• PF07478: Dala_Dala_lig_C
  • amino acids 150 to 302 (153 residues), 29.6 bits, E=2e-10
• PF02787: CPSase_L_D3
  • amino acids 426 to 502 (77 residues), 71 bits, 2.8e-23
• PF02142: MGS
  • amino acids 987 to 1087 (101 residues), 56.9 bits, E=7.7e-19

Best Hits

Swiss-Prot: 97% identical to CARB_BIFLD: Carbamoyl-phosphate synthase large chain (carB) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 66% identity to ace:Acel_1298)

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (39/46 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (13/18 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• superpathway of arginine and polyamine biosynthesis (8/17 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1127 amino acids)

>BBR_RS13635 carbamoyl-phosphate synthase large subunit (Bifidobacterium breve UCC2003)
MPKRTDIKSVMVIGSGPIVIGQAAEFDYSGTQACRVLREEGIRVILVNSNPATIMTDPEM
ADATYIEPIATPILEQIIAKERPDALLPTLGGQTALNAAMALGEAGVLKKYNVELIGASL
EAIDRGEDRELFKKVVDEAGAESARSRIAHSLEECDKIAEELGYPLVVRPSFTMGGLGSG
IAHDEEELHRIAGAGIHYSPTDEVLIEEGIEGWKEFELELMRDRNDNVVVVCPIENVDPV
GVHTGDSITVAPCFTLTDREYQKLRDIGIAIIRGVGVDTGGCNIQFAIHPKTGRIIVIEM
NPRVSRSSALASKATGFPIAKIATKLALGYTLDEIRNDITQSTPASFEPTIDYVVTKVPR
FAFEKFPGADPTLTTSMKSVGEAMALAGNFQESLGKAMRSIDKRHMGFNWDGDKPSADEV
AELLKAIHTPTEHRYLQLMRAIWGGATLDQLYAATKIDPWFLKQLLLINETAQAVREAET
LTPKLLKKAKLAGLSDVQIAHLRSLGDEGENTIRELRWNYGLKPVYKTVDTCAAEFDAAT
PYYYSCYADETELRPREREAVIILGSGPNRIGQGIEFDYTCVHAVQELGKDYDTIMVNCN
PETVSTDYDMSDRLYFEPLTFEDVLEIYEAEKKMGPVKGVIVQLGGQTPLSLAARLKAAG
VPILGTTPESIDLAENRELFGEVLKKAEMNAPRYGTALSLEEAKEAAHRIGYPVLVRPSY
VLGGRGMEIVYDDKQLNKYVDRALAEAQADTVVSGRLPSPLLIDKFLQDAIEIDVDALFD
GKELYIGGIMEHVEEAGVHSGDAACTLPPSTLSDDQIRRLREGTYAIAAGCHVQGLINVQ
YAFMANTLYVIEANPRASRTVPFASKATGVALAKAAARIMAGETIADQRANGLLLPKGDG
GDIHPGQQVAVKESVLPFKRFRTPVGKTVDILLGPEMRSTGEVMGFDRDFPHAFAKSQLA
AYDGGLPTHGNVFISVNDTDKRQLPLLAVRLEELGFKLWATEGTASVLRRYGIDSNIVDK
ISTRVDTDPEAPVEVHHAAGSVGKNVVQLIEEGKIDMILNTPNSRGSRSDGYSIRAAAIA
ADLPQFTTITEFQAALLAIEAVKHNDYQIMSIQEHSKQLFELEKQEF