Protein Info for BBR_RS13300 in Bifidobacterium breve UCC2003

Annotation: 5-(carboxyamino)imidazole ribonucleotide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01161: phosphoribosylaminoimidazole carboxylase, ATPase subunit
  • amino acids 20 to 374 (355 residues), 365.2 bits, E=2.1e-113
• PF02222: ATP-grasp
  • amino acids 125 to 297 (173 residues), 158.9 bits, E=9.8e-51
• PF17769: PurK_C
  • amino acids 323 to 374 (52 residues), 66.4 bits, 1.5e-22

Best Hits

Swiss-Prot: 45% identical to PURK_BACSU: N5-carboxyaminoimidazole ribonucleotide synthase (purK) from Bacillus subtilis (strain 168)

KEGG orthology group: K01589, 5-(carboxyamino)imidazole ribonucleotide synthase [EC: 6.3.4.18] (inferred from 100% identity to bln:Blon_1980)

Predicted SEED Role

"Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)" in subsystem De Novo Purine Biosynthesis (EC 4.1.1.21)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (39/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (18/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (21/26 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.21

Use Curated BLAST to search for 4.1.1.21 or 6.3.4.18

Protein Sequence (392 amino acids)

>BBR_RS13300 5-(carboxyamino)imidazole ribonucleotide synthase (Bifidobacterium breve UCC2003)
MPSLSEETGGRVSMLMPGSTIGIIGGGQLGRMMALSARYMGFRVGVLDPTPNCPTGQVAD
FQITAEYDDKTAIHDLAVQSDVLTYEFENVDADAIDEVRGITATPQGTDLLRVTQHRVHE
KQFINDHGTATAPWRAVNNFDELDAALDDIHYPAVLKTVSGGYDGHGQLVLRSDADLEKI
RSRSDRGGGFPPSILEGFVDFAFEASILVSGNGKDFVTFPIVRNEHRNNILHMTIAPAEV
SEHVAREAHELAIRLAKGFELAGTLAIELFITKDDQVIVNELAPRPHNSGHYTIEACSMD
QFDAHIRGIAGWPMPKPKLLSPAVMVNVLGQHVEPTRALIATHPEWNVHDYGKAEVRHDR
KMGHITVLTDNPDATVADLEATGCWDDLKKKA