Protein Info for BBR_RS13025 in Bifidobacterium breve UCC2003

Annotation: peptide chain release factor 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 TIGR00020: peptide chain release factor 2" amino acids 3 to 363 (361 residues), 473.7 bits, see alignment E=1.8e-146 PF03462: PCRF" amino acids 30 to 219 (190 residues), 199.6 bits, see alignment E=4.6e-63 PF00472: RF-1" amino acids 230 to 336 (107 residues), 129.1 bits, see alignment E=7.8e-42

Best Hits

Swiss-Prot: 67% identical to RF2_RENSM: Peptide chain release factor 2 (prfB) from Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235)

KEGG orthology group: K02836, peptide chain release factor 2 (inferred from 96% identity to bln:Blon_2027)

Predicted SEED Role

"Peptide chain release factor 2" in subsystem Programmed frameshift

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (374 amino acids)

>BBR_RS13025 peptide chain release factor 2 (Bifidobacterium breve UCC2003)
MAEFDFTQAIGEARAKYDSIAKALDVDRLKADIKDLEAQAAAPGLWDNPDNAQKITSKLS
TAESQLKRLTSASQRIDDVETLVELGQEEQDQESLDEAQNEIGEIQKDLDQMEIQTLLDG
EYDERSAVVTIRSGAGGVDAADFAQMLLRMYLRYCERNGFKAKVMDTSYAEEAGIKSATF
QVDAPYAYGRLSVEGGTHRLVRISPFDNQGRRQTSFAAVEVVPLVEATDHVDIPDTDIRV
DTYCSSGPGGQGVNTTYSAVRITHLPTGIVVTMQDERSQIQNRASAMAVLQSRLLVLRHE
EEAKKKKELAGDIKASWGDQMRSYVLHPYQMVKDLRTGYETSQTQAVFDGDINNFIEAGI
RWRHEQRRAAENEE