Protein Info for BBR_RS12995 in Bifidobacterium breve UCC2003

Annotation: 4-hydroxy-tetrahydrodipicolinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00701: DHDPS
  • amino acids 14 to 281 (268 residues), 255.8 bits, E=1.8e-80
• TIGR00674: 4-hydroxy-tetrahydrodipicolinate synthase
  • amino acids 17 to 285 (269 residues), 260.7 bits, E=5.7e-82

Best Hits

Swiss-Prot: 97% identical to DAPA_BIFLD: 4-hydroxy-tetrahydrodipicolinate synthase (dapA) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K01714, dihydrodipicolinate synthase [EC: 4.2.1.52] (inferred from 97% identity to bln:Blon_2034)

MetaCyc: 45% identical to dihydropicolinate synthase subunit (Corynebacterium glutamicum)
DIHYDRODIPICSYN-RXN [EC: 4.3.3.7]

Predicted SEED Role

"4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7)" (EC 4.3.3.7)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• L-lysine biosynthesis II (6/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.52, 4.3.3.7

Use Curated BLAST to search for 4.2.1.52 or 4.3.3.7

Protein Sequence (301 amino acids)

>BBR_RS12995 4-hydroxy-tetrahydrodipicolinate synthase (Bifidobacterium breve UCC2003)
MSEHDMHLLDPAPFGRILPAMVTPMKSDGSVDFDAAQKLAKQLVADGADGLVVNGTTGES
PVTHMDEKVNLVKAVKEVVDVPVISGAGSNDTAHTVRMVEQTQEAGADAVLVVMPYYSRP
SQDGIVGHYKAVDESAEKPIIVYDVPGRTGLKVKVNTYDRLAELEHVKAVKDATGDLAAA
VEKQQRTGLAWYSGDDGLFLPFLSIGAVGIISVIAHVASNPMQQLVQAFDRGDITTARRL
ANQLAPLVHALNGDGYQAVMAKAALKVRGVIPSTTMRLPNIGPDAAQLDKAEEGMRAAGL
L