Protein Info for BBR_RS12520 in Bifidobacterium breve UCC2003

Annotation: glycosyl hydrolase family 53 Endogalactanase galactan metabolism

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 897 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details transmembrane" amino acids 871 to 892 (22 residues), see Phobius details PF07745: Glyco_hydro_53" amino acids 53 to 429 (377 residues), 429 bits, see alignment E=2.3e-132 PF00150: Cellulase" amino acids 77 to 303 (227 residues), 28.8 bits, see alignment E=1.8e-10 PF07532: Big_4" amino acids 445 to 506 (62 residues), 46.8 bits, see alignment 3.5e-16 PF07523: Big_3" amino acids 749 to 821 (73 residues), 50.4 bits, see alignment E=4.5e-17

Best Hits

KEGG orthology group: K01224, arabinogalactan endo-1,4-beta-galactosidase [EC: 3.2.1.89] (inferred from 97% identity to blo:BL0257)

Predicted SEED Role

"Putative galactosidase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (897 amino acids)

>BBR_RS12520 glycosyl hydrolase family 53 Endogalactanase galactan metabolism (Bifidobacterium breve UCC2003)
MRRNTALTRIMASSVAAIMLCAGGMFTANAAEEEPVKAAVSVKAIQGLSDDFIGGMDVSS
MLSLEESGVTFKNANDEVEDLFTLLKESGVNYVRLRVWNDPFTADGQGYGGGNVNADRAL
TMAKRATAAGLKVLVDFHYSDFWADPSKQQVPKAWKSFEGDVDKTADTIYDYTKQTLTTF
KQAGVDVGMVQVGNETTAKIAGISGWDGMSKVFSAGSKAIREVLPEAKVVIHFTNPEKAG
TYATYAKQLSNHNVDYDVFASSYYPFWHGTTENLTSVLKNVASTYKKDVMVAETSWAYTL
DDGDDDSNTVPSKVTANNLKKYDISPQGQADEIRAVAEAVNNIGDNDGDGKNDGLGVFYW
EPAWVPVGTGGKDNAELVDTWNKYGGGWATEAAGEYDPNDAGLYWGGSGVDNQALFDFDG
KALASLPTFKYIHTGAVTDHVFTKIDPVEITATDSDSIDAIKAQLPSEVAAHYQDGVDET
ETVTWQSAALDWIRGAGTYTITGTTNAGHDVTATITVTATPAKDYVTDGNFENAENDKNW
TITGTGASITEDSGNAADGKRALKFWASDAYSFSATQTITGLEPGEYVLTAMSQGAAADN
AAIADGVTLSATAGGKTTSDALELNGWVKFDTATVPVTVGADGTATITITGNLPADAWGN
VDKVSLVKKTETPVKPSTENLDKAVAEAGKINRDECTNESLAKLDQALAAADVLLAGSAY
TEQDVNDVTKLVSDAIAGLAPKEVSSLTVTPSKTTYQVGDVIDADHDLKVVGNYSAGMGN
VTLSADQFTLDYDFSAPADAAKVTVTLKSNPNVTETYTVAVTSRAEGGSGNGSDGAGNGG
ATINPDTGEGDKTNGANGGKITGVLSNTGSAVTAVGLAVVVLGVAGGVSLALRRKRS