Protein Info for BBR_RS12325 in Bifidobacterium breve UCC2003

Annotation: type 1 glutamine amidotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00117: GATase
  • amino acids 20 to 182 (163 residues), 65.6 bits, E=4.9e-22
• PF07722: Peptidase_C26
  • amino acids 74 to 107 (34 residues), 28.4 bits, 1.4e-10

Best Hits

KEGG orthology group: None (inferred from 91% identity to bll:BLJ_0370)

Predicted SEED Role

"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (39/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (18/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (21/26 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• guanosine ribonucleotides de novo biosynthesis (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (4/6 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (5/8 steps found)
• superpathway of purine nucleotide salvage (9/14 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Protein Sequence (242 amino acids)

>BBR_RS12325 type 1 glutamine amidotransferase (Bifidobacterium breve UCC2003)
MSTPQVLILQHVPWERPGRILSTLEDIGLDTVIMNIADTKKPDLPDFGDLAGVVIMGGPM
GALDYDKYPGLKAEGKLAKAAVAVGKPVLGVCLGHQIIATALGGQLRKGDAPEIGFGPIK
RVDRHDFFSMWDKQLNVLHWHNDVVGLPEGAQLLARSSATKVQAFRLGSALGMQFHLEVC
GSLLDEWLDEPSMVKDLKAAGGSKSQLREQFAQYDQMLQPLAEQVFSGFAARCNSYSQTL
SK