Protein Info for BBR_RS12320 in Bifidobacterium breve UCC2003

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 549 TIGR00435: cysteine--tRNA ligase" amino acids 31 to 547 (517 residues), 391.4 bits, see alignment E=3.5e-121 PF01406: tRNA-synt_1e" amino acids 44 to 373 (330 residues), 345.4 bits, see alignment E=4.6e-107 PF09190: DALR_2" amino acids 411 to 471 (61 residues), 25.1 bits, see alignment E=3.2e-09

Best Hits

Swiss-Prot: 94% identical to SYC_BIFLO: Cysteine--tRNA ligase (cysS) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 95% identity to bln:Blon_0393)

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (549 amino acids)

>BBR_RS12320 cysteine--tRNA ligase (Bifidobacterium breve UCC2003)
MGNSQEPQDFNTSVMPSSLTPAHVAKAADGLNLYDTASHQVSHFVPLKPGEVGIYVCGAT
VQSSPHIGHIRAAVAFDIVRRWFLKLGYKVTFVRNVTDIDDKILDKAAAAGQRWWARAYY
YEREFTEAYNTLGVLTPTVEPRATGHMSDMIDLIQRILDNGHGYVVTDADGKPTGNVYFD
VASWPHYGELTHQKQTAEVDEAAAVADRMGPSVDATGDDKYNPVDPADASPDKHDPRDFA
LWKAPKDTDPEDARWSTPFGVGRPGWHIECSAMSHRYLGDGFDIHGGGLDLRFPHHENEM
AQTRAAGYPSAARWMHSAWVTAKGEKMSKSLGTGLSVPSVLAENSAWVVRYALGSVQYRS
MLEWSDQALVEAQAAYDRVSNFIERAGVALGGQPSREEVTGISADDLPTDFVAAMNDDVN
VSGATAAIFTTIRSGNTLLSQLADRADSEAVQAEVRETLLAVRAMLDTLGLDPLAEPWVS
AGAAGGASDGTTETPEHAALEALITEQLNARAEARKARDFAKADQIRDTLAEAGITIEDG
LQGSTWSLK