Protein Info for BBR_RS12245 in Bifidobacterium breve UCC2003

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 903 TIGR02696: guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase" amino acids 6 to 728 (723 residues), 1234.8 bits, see alignment E=0 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 18 to 728 (711 residues), 859.8 bits, see alignment E=1.6e-262 PF01138: RNase_PH" amino acids 21 to 152 (132 residues), 74.5 bits, see alignment E=2.8e-24 amino acids 355 to 487 (133 residues), 71.5 bits, see alignment E=2.4e-23 PF03726: PNPase" amino acids 268 to 350 (83 residues), 63.9 bits, see alignment E=3.9e-21 PF00013: KH_1" amino acids 591 to 647 (57 residues), 41.9 bits, see alignment 1.8e-14 PF00575: S1" amino acids 655 to 729 (75 residues), 57.8 bits, see alignment E=2.9e-19

Best Hits

Swiss-Prot: 95% identical to PNP_BIFLS: Polyribonucleotide nucleotidyltransferase (pnp) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 95% identity to bln:Blon_2270)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (903 amino acids)

>BBR_RS12245 polyribonucleotide nucleotidyltransferase (Bifidobacterium breve UCC2003)
MEGPEIKAVEAVIDNGTFGKRTLRFETGRLAQQADGAVAAYLDDDSMILSTTTAGSSPKE
NYDFFPLTVDVEEKMYAAGKIPGSFFRREGRPSSEAILACRIIDRPLRPLFPHTLRNEVQ
VVETVLAVNPDDAYDVIALNAASASTMISGLPFEGPVSGVRLALIDGQWVAFPRWSERER
AVFEIVVAGRVVENGDVAIAMIEAGAGKNAWHLIYDEGQTKPDEEVVAAGLEAAKPFIKV
ICEAQDELKKIAAKETKEFQLFPEYTDELYARIDEIAHKDLDEALSIAEKLPRQDRIHEI
KEHVREVLADEFTDMDDAEKDKELGNAFKELQRQIVRRRILTEDYRIDGRGLRDIRTLSA
EVDIVPRVHGSALFQRGETQILGVTTLNMLKMEQQIDALSGPQSKRYMHNYEMPPYSTGE
TGRVGSPKRREIGHGALAEKALVPVLPSREDFPYAIRQVSEAIGSNGSTSMGSVCASTLS
LLAAGVPLKAPVAGIAMGLVSGDVDGQHIFKTLTDILGAEDAFGDMDFKVAGTSEFITAL
QLDTKLDGIPADILAAALQQAKEARATILEVINECIDGPAEMSEFAPRIITTTVPVEKIG
EVIGPKGKMINQIQEDTGAEIAIEDDGTVFISSEGGEAAEKAKSIIDSIANPHVPEAGET
YNGKVVKTTSFGAFVNLTPGTDGLLHISQIRNLANGERIDAVEDVLKEGDTVEVVVQGVD
DRGKISLAIPGFEDQENNAGSRGGRGDRDDHRGSRGRDDRRGGGRGRRSDRDDRDFDDRD
DRPRRRRSDDFEDDYEDRPRRRRSDDRDFDRDDRDSRDEDRPRRRRSADFEDDYEDRPRR
RRSDDRGDRDDRRGGSRGGRGRGSDRNPRYATDDNYDDYRADREERSERPRRRVRRDFDP
FED