Protein Info for BBR_RS11705 in Bifidobacterium breve UCC2003
Annotation: Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 99% identical to GATA_BIFLS: Glutamyl-tRNA(Gln) amidotransferase subunit A (gatA) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 98% identity to blb:BBMN68_1143)Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit A (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- L-citrulline biosynthesis (5/8 steps found)
- superpathway of L-citrulline metabolism (7/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-positive bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (513 amino acids)
>BBR_RS11705 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA (Bifidobacterium breve UCC2003) MSNETATLVKLSAAEQAAAIKKGDVTSRELVEAHLKVIEAAEPSIKAFLKVSGDVALEQA DAFDAKSAEDKAVLPELAGVPIAIKDMIVTKGIETTAASKILEGWVPPYDATVIEKLKAA GMPILGKTNLDEFAQGSSTEHSAYQTTHNPWDTERVPGGSGGGSASAVAAFEAPLALGTD TGGSIRQPGALTGTVGVKPTYGGVSRFGAIAMASSLDQIGPVSRTVLDSALLQEIIGGHD KRDSTSIPEGPRPMVAAAREGAKRDLKGMKVGLIKELGGDGFQPGVEARFNEAVDKLKEM GAEVVEVSCPHIGYSLGAYYIIMPSEVSSNLARYDGMRYGLRVMPPAGVPQTAANMMAYT REAGFGDEVKRRIILGTYALSAGYYDAWYGSAQKVRTLIIEDFKKAFEQVDVLISPTSPS TAFKFGEKMDDPLAMYVNDIATIPANLAGMPAMSIPAGLSDDGLPVGFQFIAPQQRDEVM YKPAAALEAALEDSWNGPIWKDLKTPWLDGLGK