Protein Info for BBR_RS10900 in Bifidobacterium breve UCC2003

Annotation: molecular chaperone DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 626 TIGR02350: chaperone protein DnaK" amino acids 4 to 582 (579 residues), 917.5 bits, see alignment E=1.6e-280 PF00012: HSP70" amino acids 4 to 78 (75 residues), 98.9 bits, see alignment E=2.3e-32 amino acids 79 to 582 (504 residues), 706.9 bits, see alignment E=1.8e-216 PF06723: MreB_Mbl" amino acids 4 to 355 (352 residues), 51.3 bits, see alignment E=8e-18

Best Hits

Swiss-Prot: 98% identical to DNAK_BIFLD: Chaperone protein DnaK (dnaK) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 98% identity to blj:BLD_1313)

MetaCyc: 55% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (626 amino acids)

>BBR_RS10900 molecular chaperone DnaK (Bifidobacterium breve UCC2003)
MARAVGIDLGTTNSCIATLEGGEPTVIVNAEGMRTTPSVVAFSKSGEILVGEVAKRQAVT
NVDRTISSVKRHMGTDWTVDIDGKKWTPQEISAQILMKLKRDAEAYLGEPVTDAVITCPA
YFNDAQRQATKDAGKIAGLNVLRIINEPTAAALAYGLEKGKEDERILVFDLGGGTFDVSL
LEIGKDDDGFSTIQVQATNGDNHLGGDDWDQKVIDWLVGEVKNKYGVDLSKDKIALQRLK
EAAEQAKKELSSSTSTSISMQYLAMTPDGTPVHLDETLTRAHFEEMTSDLLGRCRTPFNN
VLHDAGISVSDIDHVVLVGGSTRMPAVKDLVKELTGGKEANQSVNPDEVVAVGAAVQSGV
IKGDRKDVLLIDVTPLSLGIETKGGIMTKLIDRNTAIPTKRSEVFSTAEDNQPSVLIQVY
QGEREFARDNKPLGTFELTGIAPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGKEQSMTI
TGGSGLPKDEIDRMVKEAEAHEAEDKQRKEDAETRNQAEAFAYSTEKLVNDNKDKLSDDI
VKEVTDKVNALKEALKGDDTDKVKTAQGELMTSAQKIGQVLYAQQGAEGAAAGAGAAGAS
AGSASGSDDDTVEAEVVDDDDDKDNK