Protein Info for BBR_RS10780 in Bifidobacterium breve UCC2003

Annotation: sucrose phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 TIGR03852: sucrose phosphorylase" amino acids 3 to 466 (464 residues), 427.9 bits, see alignment E=2.1e-132 PF00128: Alpha-amylase" amino acids 35 to 205 (171 residues), 40.1 bits, see alignment E=3.6e-14 PF09244: Suc_Porlyase_C" amino acids 435 to 503 (69 residues), 109.1 bits, see alignment E=1.2e-35

Best Hits

Swiss-Prot: 91% identical to SUCP_BIFAA: Sucrose phosphorylase (sucP) from Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)

KEGG orthology group: K00690, sucrose phosphorylase [EC: 2.4.1.7] (inferred from 98% identity to bll:BLJ_0100)

MetaCyc: 95% identical to sucrose phosphorylase (Bifidobacterium longum)
Sucrose phosphorylase. [EC: 2.4.1.7]

Predicted SEED Role

"Sucrose phosphorylase (EC 2.4.1.7)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Sucrose utilization or Sucrose utilization Shewanella (EC 2.4.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.7

Use Curated BLAST to search for 2.4.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (508 amino acids)

>BBR_RS10780 sucrose phosphorylase (Bifidobacterium breve UCC2003)
MKNKVQLIAYADRLGDGTLSSMTDILRTRFDGVYDGVHVLPFFTPFDGADAGFDPIDHTK
VDPRLGSWDDVAELSKTHDIMVDAIVNHMSWESAQFQDVLKNGEHSEYYPMFLTMSSVFP
NGATEEDLAGIYRPRPGLPFTHYKFAGKTRLVWVSFTPQQVDIDTDSAKGWEYLMSIFDQ
MAASHVRYIRLDAVGYGAKEAGTSCFMTPKTFKLISRLREEGVKRGLEILIEVHSYYKKQ
VEIASKVDRVYDFALPPLLLHSLFTGHVEPVVHWTEIRPNNAVTVLDTHDGIGVIDIGSD
QLDRSLKGLVPDEDVDNLVNTIHANTHGESQAATGAAASNLDLYQVNSTYYSALGCNDQH
YLAARAVQFFLPGVPQVYYVGALAGRNDMELLRKTNNGRDINRHYYSTAEIDENLERPVV
KALNALAKFRNELPAFNGEFSYEADGDTSITFRWIAADGKTKAALIFEPGRGLGTDNTTP
VASLAWTDAAGDHETDDLLSNPPIADID