Protein Info for BBR_RS10755 in Bifidobacterium breve UCC2003

Annotation: adenylosuccinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 TIGR00184: adenylosuccinate synthase" amino acids 5 to 424 (420 residues), 561.5 bits, see alignment E=5.7e-173 PF00709: Adenylsucc_synt" amino acids 5 to 421 (417 residues), 621.2 bits, see alignment E=3.9e-191

Best Hits

Swiss-Prot: 97% identical to PURA_BIFLS: Adenylosuccinate synthetase (purA) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)

KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 97% identity to bll:BLJ_0090)

MetaCyc: 48% identical to adenylosuccinate synthetase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate synthase. [EC: 6.3.4.4]

Predicted SEED Role

"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>BBR_RS10755 adenylosuccinate synthase (Bifidobacterium breve UCC2003)
MPGIVLIGAQWGDEGKGKATDLIGTKVDYVARFNGGNNAGHSVVVGDESYALHLLPSGII
NPNLTPVIGNGVVVDPEVLFEEIDGLESRGIDCSHLLVSEAAHIIAPYHRTLDKVTERFL
GKHKIGTTGRGIGPAYADKINRIGIRVHDLFNADHLHDKVEASLHQKNQMLVKLYNRRAI
DVDRTTDELLKLGERLKPYVANTGLILNKALDEGKTVLFEGAQATMLDVDHGTYPFVTSS
NPTAGGACTGTGVGPTKITRVIGVAKAYVTRVGEGPFPTELLDKSGEWLRQQGHEFGVTT
GRPRRCGWFDAVVNRYASQVNGLTDIVLTKLDVLTGLKEIPICVAYDVDGERHDDMPTDQ
AAFAAAKPIYETMPGWNEDISNCHSFDDLPATCQAYVKRLEELSNCRISVIGTGPQRDHV
IQINSLVD