Protein Info for BBR_RS10550 in Bifidobacterium breve UCC2003

Annotation: tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00579: tRNA-synt_1b
  • amino acids 38 to 316 (279 residues), 172.9 bits, E=4.9e-55
• TIGR00233: tryptophan--tRNA ligase
  • amino acids 38 to 361 (324 residues), 249.4 bits, E=2.8e-78

Best Hits

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 99% identity to bln:Blon_0064)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

• tRNA charging (19/21 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Protein Sequence (363 amino acids)

>BBR_RS10550 tryptophan--tRNA ligase (Bifidobacterium breve UCC2003)
MTDEQQLTAAGNEMSASFLAAKKRSDETLAKLEAKPSSFTMLTGDRPTGRLHLGHYFGSI
RERVAMQERGVNTNIIIADYQVITDRDTTEHIQDNVLNLVLDYMAAGIDPNKTMMFTHSA
VPAENQLLLPFLSLVTEAELHRNPTVKSEMEASGHALTGLLLTYPVHQACDILFCKANVV
PIGKDNLPHVEITRTIARRFNERYAKKNPVFPEPAAILSEAPEIPGLDGRKMSKSYGNSI
MLGATAEETAKLIKKSPTDSERRITFDPVARPQVSALLTTAGLVTGRDPKDIAEEIGDSG
AGALKAYVIESVNEFLAPHRERRAELAKDMDSIRDILHDGNKRANAIAEETLDQVRDAMG
MKY