Protein Info for Ac3H11_549 in Acidovorax sp. GW101-3H11

Annotation: O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 34 to 455 (422 residues), 638.2 bits, E=2.6e-196
• PF01053: Cys_Met_Meta_PP
  • amino acids 35 to 455 (421 residues), 462 bits, E=2.3e-142
• PF01041: DegT_DnrJ_EryC1
  • amino acids 87 to 210 (124 residues), 38.7 bits, E=1.5e-13
• PF00155: Aminotran_1_2
  • amino acids 92 to 250 (159 residues), 28 bits, E=2.6e-10
• PF00266: Aminotran_5
  • amino acids 125 to 241 (117 residues), 26.5 bits, E=6.3e-10

Best Hits

Swiss-Prot: 54% identical to FUB7_FUSO4: Sulfhydrylase FUB7 (FUB7) from Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 89% identity to ajs:Ajs_2286)

MetaCyc: 48% identical to Met17 (Saccharomyces cerevisiae)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (3/6 steps found)
• homocysteine and cysteine interconversion (1/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See A0A165LJH2 at UniProt or InterPro

Protein Sequence (458 amino acids)

>Ac3H11_549 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) (Acidovorax sp. GW101-3H11)
MARAIGTSRNAQPWVEADCPTPFPSTKETSFMKIETLAVHAGYSPDPTTKAVAVPIYQTV
AYAFDSAQHGADLFDLKVPGNIYTRIMNPTTDVLEKRVAALEGGIAALAVASGMAAITYA
IQTIAEAGDNIVSASTLYGGTYNLFAHTLPQQGITTRFADPRDPASFAQHIDARTKAIFI
ESIGNPLGNVTDIAAIAKVAHDHGVPLIVDNTVPSPYLLRPIEHGADIVVHSLTKYLGGH
GNSVGGAIVDSGKFPWAEHKARFPRLNEPDVSYHGVVYTEALGPAAFIGRARVVPLRNTG
AALSPQSAFLILQGIETLALRMDRICDNTLALAKYLQSHPKVEWVRYAGLPDHPDHALVQ
RQSGGKASGILSFGLKSTDADPRAAGARFLDALQLFTRLVNIGDAKSLATHPASTTHRQL
NPEELAKAGVSESMVRLSVGIEHIDDLRADLVQALDAV