Protein Info for Ac3H11_4805 in Acidovorax sp. GW101-3H11

Annotation: 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 PF00171: Aldedh" amino acids 28 to 477 (450 residues), 497.5 bits, see alignment E=1.6e-153

Best Hits

KEGG orthology group: K00151, 5-carboxymethyl-2-hydroxymuconic-semialdehyde dehydrogenase [EC: 1.2.1.60] (inferred from 81% identity to aaa:Acav_3012)

Predicted SEED Role

"5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic amino acid degradation or Central meta-cleavage pathway of aromatic compound degradation (EC 1.2.1.60)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.60

Use Curated BLAST to search for 1.2.1.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165L9H1 at UniProt or InterPro

Protein Sequence (491 amino acids)

>Ac3H11_4805 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60) (Acidovorax sp. GW101-3H11)
LSTDTLNVAGIAVSPHHYIDGQRVASDMRFDLHSPIDQALLGRISEGSPAHVDAAVSAAA
RAFPAWSALTAAERKPYLDRFAAEIGKRAEAFCTLESNDAGVLLSRMKHGVVPRAMLNIT
WFAEHALSLQDRPIETEQATHLVRHDPAGVVAIITPWNAPLMLATWKLGPALAAGNCVIV
KPPEWAPLTSSLLADCAHAAGLPPGVFNIVQGAGVSTGARLVSDPRLARISFTGSVPTAK
WIAQSAGANLVPCSLELGGKSAFIVLEDADIDNAAATGALMYRNAGQVCLAGTRFLVHRK
VHDAFVAALRGYVEKLTVGDPRDGATEVGPIIHPRQVERVHGFVQRAVADGATLLWGGAQ
HPFGAQYYQPTLLTDVRQDSEIVQNEVFGPVLTLQTFDSDEEAIALANGTDYGLGGVCYG
ATEHASAVAQQVRTGFIWVNSFGIRDLAAPFGGIKRSGVGREGGDWSFEFFCDVKDVVVP
KKPFRASFSHR