Protein Info for Ac3H11_4685 in Acidovorax sp. GW101-3H11

Annotation: Holliday junction DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 PF05496: RuvB_N" amino acids 33 to 191 (159 residues), 272.8 bits, see alignment E=2.4e-85 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 37 to 339 (303 residues), 485.6 bits, see alignment E=2.4e-150 PF07728: AAA_5" amino acids 67 to 185 (119 residues), 33 bits, see alignment E=1.7e-11 PF00004: AAA" amino acids 68 to 190 (123 residues), 74.9 bits, see alignment E=2.6e-24 PF17864: AAA_lid_4" amino acids 194 to 267 (74 residues), 113.1 bits, see alignment E=1.2e-36 PF05491: RuvB_C" amino acids 269 to 338 (70 residues), 81.9 bits, see alignment E=7.5e-27

Best Hits

Swiss-Prot: 92% identical to RUVB_ACIAC: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 92% identity to aaa:Acav_4120)

MetaCyc: 71% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165LS62 at UniProt or InterPro

Protein Sequence (354 amino acids)

>Ac3H11_4685 Holliday junction DNA helicase RuvB (Acidovorax sp. GW101-3H11)
MTIQTDDFAPAPTKRVVSAAPSSPQEEAIERALRPKLLQEYVGQAKAREQLEIFIGAARK
REEALDHVLLFGPPGLGKTTLSHIIAAELGVNLRQTSGPVLEKPKDLAALLTNLEKNDVL
FIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKLDLQPFTLVGATTRAGMLTNP
LRDRFGIVARLEFYTAEELARIVKRSAGLLNAPMDDEGGFEIARRSRGTPRIANRLLRRV
RDYAEVKGDGRITKPIADKALAMLDVDPQGFDVMDRKLLEAVIHRFDGGPVGLDNIAASI
GEEAGTIEDVIEPYLIQQGFLQRTPRGRIATLAAFRHLGLQPPAQRGNPDLFGA