Protein Info for Ac3H11_435 in Acidovorax sp. GW101-3H11

Annotation: Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01368: carbamoyl-phosphate synthase, small subunit
  • amino acids 30 to 403 (374 residues), 464.2 bits, E=1.4e-143
• PF00988: CPSase_sm_chain
  • amino acids 32 to 157 (126 residues), 173.6 bits, E=2.4e-55
• PF00117: GATase
  • amino acids 224 to 399 (176 residues), 180.3 bits, E=5e-57
• PF07722: Peptidase_C26
  • amino acids 278 to 323 (46 residues), 22.5 bits, 1.3e-08

Best Hits

Swiss-Prot: 78% identical to CARA_RUBGI: Carbamoyl-phosphate synthase small chain (carA) from Rubrivivax gelatinosus (strain NBRC 100245 / IL144)

KEGG orthology group: K01956, carbamoyl-phosphate synthase small subunit [EC: 6.3.5.5] (inferred from 89% identity to dia:Dtpsy_1456)

Predicted SEED Role

"Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of arginine and polyamine biosynthesis (15/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See A0A165LKA3 at UniProt or InterPro

Protein Sequence (405 amino acids)

>Ac3H11_435 Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) (Acidovorax sp. GW101-3H11)
VCAGGLQAPGDRNHPENPVLLSLQGSFPPAILALADGTVFIGNSIGATGTTVGEVVFNTA
ITGYQEILTDPSYCQQIVTLTYPHIGNYGVNAEDIEADKVHAAGLIIKDLPLLASNFRST
ETLSQYLVREKTVAIANIDTRKLTRLLRTQGAQNGAIVGLAAGQVVTQGLIDEAIAKAKA
APNMAGLDLAKVVTTASRYEWTQTEWQLGSGYGVLAQPRFHVVAYDFGVKKNILRMLAER
GCKVTVVPAQTPAAEVLAMNPDGVFLSNGPGDPAPCDYAIEATRQIIDAGVPTFGICLGH
QIMALASGATTFKMDNSHHGANHPVKDLDNGRVSITSQNHGFAVDMASLPATLRATHISL
FDGTLQGLARTDKPAFCFQGHPEASPGPHDIAYLFDRFTALMEKK