Protein Info for Ac3H11_4257 in Acidovorax sp. GW101-3H11

Annotation: DNA gyrase subunit B (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 868 TIGR01059: DNA gyrase, B subunit" amino acids 34 to 868 (835 residues), 939.3 bits, see alignment E=5.7e-287 PF02518: HATPase_c" amino acids 61 to 215 (155 residues), 56.9 bits, see alignment E=8.2e-19 PF00204: DNA_gyraseB" amino acids 266 to 445 (180 residues), 188.7 bits, see alignment E=1.9e-59 PF01751: Toprim" amino acids 474 to 593 (120 residues), 70.6 bits, see alignment E=3.1e-23 PF21249: GyrB_hook" amino acids 595 to 623 (29 residues), 27.2 bits, see alignment (E = 7.1e-10) PF18053: GyrB_insert" amino acids 636 to 794 (159 residues), 141.7 bits, see alignment E=6.7e-45 PF00986: DNA_gyraseB_C" amino acids 798 to 857 (60 residues), 105.1 bits, see alignment 4.9e-34

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 86% identity to aaa:Acav_0003)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165K1L4 at UniProt or InterPro

Protein Sequence (868 amino acids)

>Ac3H11_4257 DNA gyrase subunit B (EC 5.99.1.3) (Acidovorax sp. GW101-3H11)
MTADNNLPEQEPTGTGEPVPHKIDTNQAGASESYGEGSITILEGLEAVRKRPGMYIGDTS
DGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHTDNSISVTDNGRGIPTGVKMDDKHEPK
RSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKWLRLTVRREGKVHQIEFAR
GFVQDRLLDKVDGFEVSPMKVTGETEKRGTEVHFLPDTEIFKENYDFHYEILAKRLRELS
FLNNGVRIRLKDERSGKEDDFSGAGGVRGFVEFINKGKTVLHPTSFYAAGERPAETYGGI
PGTHIGVEVSMQWNSAYTEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIEENELAKK
AKVEVTGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEDIVGKLLTDYLQERP
ADAKIICGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPAMCEIYIVEG
DSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKAGGTT
GGDDFDVAKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVK
AGKEELYLKDGPALDGFLLRIALNHASVSTGGANPQVLAGDTLAELARKHQVAESVIHRL
SNFMDQEALRAVADGVSLKLDTVAEAEASAVALQIKLRELNTTGTPAEVAGEFDARTDKP
ILRISRRHHGNVKSSVITQDFVHGADYAALAEAADTFRGLLGEGAKALRGEGDKQKEEKV
GDFRQAMKWLISEAERTTSRQRYKGLGEMNPEQLWETTMDPNVRRLLRVQIDDAIEADRV
FTMLMGDEVEPRRDFIETNALRAGNIDV