Protein Info for Ac3H11_3410 in Acidovorax sp. GW101-3H11

Annotation: Trans-feruloyl-CoA synthase (EC 6.2.1.34)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 50 to 435 (386 residues), 164.6 bits, E=1.6e-52

Best Hits

KEGG orthology group: K12508, feruloyl-CoA synthase [EC: 6.2.1.34] (inferred from 69% identity to vap:Vapar_4403)

MetaCyc: 53% identical to 4-coumarate-CoA ligase (Rhodopseudomonas palustris CGA009)
4-coumarate--CoA ligase. [EC: 6.2.1.12]

Predicted SEED Role

"Trans-feruloyl-CoA synthase (EC 6.2.1.34)" (EC 6.2.1.34)

MetaCyc Pathways

• 4-coumarate degradation (aerobic) (4/5 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• umbelliferone biosynthesis (2/3 steps found)
• ferulate degradation (1/3 steps found)
• 4-coumarate degradation (anaerobic) (3/6 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• naringenin biosynthesis (engineered) (1/4 steps found)
• xanthohumol biosynthesis (1/4 steps found)
• chlorogenic acid biosynthesis II (1/5 steps found)
• flavonoid biosynthesis (1/5 steps found)
• phaselate biosynthesis (1/5 steps found)
• rosmarinic acid biosynthesis I (3/10 steps found)
• chlorogenic acid biosynthesis I (1/8 steps found)
• avenanthramide biosynthesis (1/9 steps found)
• superpathway of rosmarinic acid biosynthesis (4/14 steps found)
• curcuminoid biosynthesis (1/10 steps found)
• coumarins biosynthesis (engineered) (3/13 steps found)
• flavonoid di-C-glucosylation (3/15 steps found)
• monolignol biosynthesis (1/15 steps found)
• suberin monomers biosynthesis (3/20 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Phenylpropanoid biosynthesis
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.34

Use Curated BLAST to search for 6.2.1.12 or 6.2.1.34

Sequence Analysis Tools

See A0A165KH39 at UniProt or InterPro

Protein Sequence (622 amino acids)

>Ac3H11_3410 Trans-feruloyl-CoA synthase (EC 6.2.1.34) (Acidovorax sp. GW101-3H11)
MTANLPSPRYRPLRFGVTEVTTRQGDNGVVYVRATQDLQPHPVRLTDRLAHWAQTTPDRT
WMAQRVKNADGSLGDWRHISYREAWDSARRIAQALLDRQLSADRPVAILSENDLDHALLS
LGCLVAGVPQCTVSSAYSTVSKDYDKLRHAIDTLTPGLVFASNAERYAAAINATVGTDVH
VVLGQGTLPGRSTTPFSALLATEATPAVDAAHAAITGESIAKFMFTSGSTKAPKAVIITH
GMWAANLQQMNQSMPVLVEQDLVLVDWLPWNHTFGGNHNFGIVIYNGGTLYLDEGKPTPT
GMVETIRNLREIAPTVYFNVPTGFEVIAHTMKTDAQLRKNLLSRVQMFFFAAAALSQPIW
DSLFESAEREVGERIVMNTGLGMTEGCPFCVAVNSPNVQAGDIGVPTPGLELKLVPVDGK
VEVRYRGPNITQGYWRNAEATQEAFDEEGFYCSGDAVKWIDENDLNKGLKFDGRIAEDFK
LSTGTFVSVGPMRAKVIAGCAPYVQDAVITGINLKEVGALLIPTQSIRALAGLPDGASLQ
QVLESAPVQQHFQTVINQLAAQATGSATRVARAHLMAEPPSLDKGEITDKGSINQRAVLK
HRADLVNALHDGSLPFTLVPHH