Protein Info for Ac3H11_3406 in Acidovorax sp. GW101-3H11

Annotation: Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01144: CoA_trans
  • amino acids 5 to 240 (236 residues), 112.6 bits, E=9.1e-37

Best Hits

Swiss-Prot: 48% identical to CARA_ACEWO: Caffeate CoA-transferase (carA) from Acetobacterium woodii

KEGG orthology group: None (inferred from 60% identity to tmz:Tmz1t_2487)

MetaCyc: 52% identical to Pct (Anaerotignum propionicum)
Propionate CoA-transferase. [EC: 2.8.3.1]; 2.8.3.1 [EC: 2.8.3.1]

Predicted SEED Role

"Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 2.8.3.8)

MetaCyc Pathways

• propanoate fermentation to 2-methylbutanoate (5/6 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• (R)-lactate fermentation to propanoate II (acrylate pathway) (2/4 steps found)
• acetyl-CoA fermentation to butanoate (4/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (3/6 steps found)
• pyruvate fermentation to acetone (2/5 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
• succinate fermentation to butanoate (2/7 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• gallate degradation III (anaerobic) (4/11 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Styrene degradation

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.8

Use Curated BLAST to search for 2.8.3.1 or 2.8.3.8

Sequence Analysis Tools

See A0A165KH11 at UniProt or InterPro

Protein Sequence (530 amino acids)

>Ac3H11_3406 Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (EC 2.8.3.8) (Acidovorax sp. GW101-3H11)
MKIITAAQAGALIPDNATIFLGGLAVSSLPEEVLKGIEKNFLDHGSPKNVTTWACGAIGN
SKDAGMVHFAHPGMIKRTVAGHFGQTGAEMMKMVFDGEVEAYNFPQGSLSHLTRHIASRS
PGLLTKVGLGTFVDPRIEGGKLNSKSTEDLVKLVEFNGEEWLFYPAPKVDVAIIRGTLAD
ENGNITLDKEGVLLEQINIAHAAKACGGIVIAQVERIVKAGSLHPKSVKVPGVSVDYVVV
SQPENHMQTITTQFNAALCGDVRVPVNSLAPMALDERKVIARRTALELKPGAITNLGIGI
PAGVPSVAAEEGVADQFTLSVESGITGGIPAQLGDFGTAYNADAIIEQSLQFDFYDGGGL
DASFLGLAQADNHGNVNVSKFNGRPVGCGGFVNITRSTKNLVFCGTFTAGGLEVKVADGQ
LTIVKEGRSRKFIEKVEQITFNGLDAAQREQNVLFVTERAVFHLTTEGLELTEIAPGIDL
ERDVLAHMGFKPIMRNVKTMDAGLFHEKWGGLAAAMAAKSEAVAQAAAGQ