Protein Info for Ac3H11_338 in Acidovorax sp. GW101-3H11

Annotation: Glucarate dehydratase (EC 4.2.1.40)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 TIGR03247: glucarate dehydratase" amino acids 15 to 454 (440 residues), 849.4 bits, see alignment E=2.5e-260 PF13378: MR_MLE_C" amino acids 198 to 413 (216 residues), 164.2 bits, see alignment E=1.9e-52

Best Hits

Swiss-Prot: 74% identical to GUDD_PSEPU: Glucarate dehydratase (gudD) from Pseudomonas putida

KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 84% identity to aav:Aave_3623)

MetaCyc: 74% identical to D-glucarate dehydratase subunit (Pseudomonas putida)
Glucarate dehydratase. [EC: 4.2.1.40]

Predicted SEED Role

"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165LES6 at UniProt or InterPro

Protein Sequence (454 amino acids)

>Ac3H11_338 Glucarate dehydratase (EC 4.2.1.40) (Acidovorax sp. GW101-3H11)
LLLPTMTTSTPSTSSTPVITELRVVPVAGHDSMLMNLSGAHGPFFTRNLIILRDSAGHTG
VGEVPGGEKIRQTIEDARALIVGCPIGHHNAVLNAMRAQFADRDAGGRGLQTFDLRITIH
AVTAVESAFLDLLGQHLGVPVAALLGEGVQRESVQMLGYLFYVGDRAKTDLAYRTEPGAD
NDWFRLRNEKAMTAEGIVRLAEAAYERYGFADFKLKGGVLRGEEEVEAIRALHQRFPEAR
VTLDPNGGWLLKDAIRITRDLHGVMAYAEDPCGAEGVFSGREVMAEFRRATGLPTATNMV
ATDWREMAHSLALQSVDIPLADPHFWTMAGSVRVAQMCQAFGLTWGSHSNNHFDVSLAMF
THVGAAAPGRVTAIDTHWIWQDGERLTKEPFQIKNGFIDLPKKPGLGVELDMDEVERAHR
LYLQHGLGARDDAIAMQYLIPGWKFNNKSPCMVR