Protein Info for Ac3H11_2994 in Acidovorax sp. GW101-3H11

Updated annotation (from data): 3-ketoacyl-CoA thiolase (EC 2.3.1.16)
Rationale: Specifically important for utilizing L-Isoleucine. Automated validation from mutant phenotype: the predicted function (METHYLACETOACETYLCOATHIOL-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: 3-ketoacyl-CoA thiolase (EC 2.3.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 53% identical to ATOB_ECOLI: Acetyl-CoA acetyltransferase (atoB) from Escherichia coli (strain K12)

KEGG orthology group: K00626, acetyl-CoA C-acetyltransferase [EC: 2.3.1.9] (inferred from 87% identity to aav:Aave_4368)

MetaCyc: 67% identical to acetyl-CoA C-acetyltransferase (Methylibium petroleiphilum PM1)
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16, 2.3.1.9]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16)" in subsystem Biotin biosynthesis or Isoleucine degradation or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle or n-Phenylalkanoic acid degradation (EC 2.3.1.16)

MetaCyc Pathways

oleate β-oxidation (29/35 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
ketolysis (3/3 steps found)
polyhydroxybutanoate biosynthesis (3/3 steps found)
L-isoleucine degradation I (5/6 steps found)
fatty acid salvage (5/6 steps found)
propanoate fermentation to 2-methylbutanoate (5/6 steps found)
pyruvate fermentation to butanol II (engineered) (5/6 steps found)
acetoacetate degradation (to acetyl CoA) (2/2 steps found)
valproate β-oxidation (7/9 steps found)
4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
2-methyl-branched fatty acid β-oxidation (10/14 steps found)
1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
ketogenesis (3/5 steps found)
(2S)-ethylmalonyl-CoA biosynthesis (2/4 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
acetyl-CoA fermentation to butanoate (4/7 steps found)
3-phenylpropanoate degradation (6/10 steps found)
5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
glycerol degradation to butanol (10/16 steps found)
pyruvate fermentation to acetone (2/5 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
pyruvate fermentation to butanol I (4/8 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
pyruvate fermentation to butanoate (3/7 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (4/9 steps found)
ethylbenzene degradation (anaerobic) (1/5 steps found)
fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
isopropanol biosynthesis (engineered) (1/5 steps found)
2-deoxy-D-ribose degradation II (3/8 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (4/10 steps found)
10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
4-ethylphenol degradation (anaerobic) (1/6 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
2-methylpropene degradation (2/8 steps found)
L-tryptophan degradation III (eukaryotic) (7/15 steps found)
mevalonate pathway I (eukaryotes and bacteria) (1/7 steps found)
mevalonate pathway II (haloarchaea) (1/7 steps found)
4-oxopentanoate degradation (2/9 steps found)
isoprene biosynthesis II (engineered) (1/8 steps found)
mevalonate pathway III (Thermoplasma) (1/8 steps found)
mevalonate pathway IV (archaea) (1/8 steps found)
ethylmalonyl-CoA pathway (3/11 steps found)
L-lysine fermentation to acetate and butanoate (2/10 steps found)
methyl tert-butyl ether degradation (2/10 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
L-glutamate degradation VII (to butanoate) (3/12 steps found)
10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
jasmonic acid biosynthesis (5/19 steps found)
toluene degradation VI (anaerobic) (4/18 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
platensimycin biosynthesis (7/26 steps found)
Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
sitosterol degradation to androstenedione (1/18 steps found)
androstenedione degradation I (aerobic) (6/25 steps found)
superpathway of ergosterol biosynthesis I (5/26 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
superpathway of testosterone and androsterone degradation (6/28 steps found)
androstenedione degradation II (anaerobic) (4/27 steps found)
superpathway of L-lysine degradation (10/43 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
superpathway of cholesterol biosynthesis (5/38 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165JRF5 at UniProt or InterPro

Protein Sequence (397 amino acids)

>Ac3H11_2994 3-ketoacyl-CoA thiolase (EC 2.3.1.16) (Acidovorax sp. GW101-3H11)
MSTTIQDPIVIVGAARTPMGSLQGDFSSLAAHDLGGAAIKAAIERAGVSPDAVGEVLFGN
CLMAGQGQAPARQAAFKGGLPKGAGAVTLSKMCGSGMKAAMMAHDMLLAGSHDVMVAGGM
ESMTNAPYLLQKGRGGYRLGHDRIFDHMMLDGLEDAYEAGRSMGTFGEDCAAKYSFTREQ
QDAFATASVQRAKAATESGAFAAEIVPVTVKTRAGETVVSVDEGPGKVKLEKIATLKPAF
KKDGTITAASSSSINDGAAALVMMRESTAKKLGAKPLARIVSHATHAQEPEWFATAPLGA
TQKALAKAGWQVGDVQLWEINEAFAVVPMALMKELDLPHDKVNVNGGACALGHPIGASGA
RIMVTLIHALKARGLTKGLATLCIGGGEATAVALELV