Protein Info for Ac3H11_27 in Acidovorax sp. GW101-3H11

Annotation: Glutaminyl-tRNA synthetase (EC 6.1.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 612 PF00749: tRNA-synt_1c" amino acids 65 to 387 (323 residues), 345 bits, see alignment E=5.2e-107 TIGR00440: glutamine--tRNA ligase" amino acids 66 to 612 (547 residues), 650.5 bits, see alignment E=1.2e-199 PF03950: tRNA-synt_1c_C" amino acids 391 to 496 (106 residues), 67 bits, see alignment E=2.2e-22 PF20974: tRNA-synt_1c_C2" amino acids 517 to 589 (73 residues), 78.5 bits, see alignment E=7.4e-26

Best Hits

Swiss-Prot: 64% identical to SYQ_BORPE: Glutamine--tRNA ligase (glnS) from Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 83% identity to aaa:Acav_2833)

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165HYI3 at UniProt or InterPro

Protein Sequence (612 amino acids)

>Ac3H11_27 Glutaminyl-tRNA synthetase (EC 6.1.1.18) (Acidovorax sp. GW101-3H11)
MSTPSPANTPASAAAADTAESTKPSNFLRQIIEKDLEQGTYATRRWAGAPGDAAHHAQGE
PDPAKIRTRFPPEPNGYLHIGHAKSICINFGLARDYGGVCHLRFDDTNPEKEDTEYVNSI
IDAVKWLGFDWAQLGQAPGSTAPYQASDYFGFMYRAAEYLIEAGHAYVDEQTTEQMRANR
GDFSKPGVDSPFRTRTPEENLRIFREMKGGEHEDGSMVLRAKIDMASPNINMRDPAIYRI
RRATHHNTGDTWCIYPMYTYAHPIEDALEQITHSLCTLEFEDQRPFYDWLLERLTEGGLL
ASPPPRQYEFARLNLTYVITSKRKLAQLVYDHKVSGWDDPRMPTIVGLRRRGYTPEAVQV
FAERIGVTKSDSWIDYSTLEGCLREDLELKAHRGMAVLNPVKLVLTNWDDVMGAGHLEPC
SLPALPHPPEGVESPVRHFTIGKEVWIEREDFEEVPPKGYKRLFPGNKVRLKGGYVIECT
GCTKDANGVITEVLATVVPDTKSGTPGADSVKVKAAITWVGVADGVNAEVRMYDRLFLDA
HPDAGGKDFIESLNPNSLKVVTAIVEPSLANAQPDDKFQFERHGYFVADRIDHKAEKPVF
NLAVGLKDSWGK