Protein Info for Ac3H11_2314 in Acidovorax sp. GW101-3H11

Annotation: DNA ligase (EC 6.5.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 PF01653: DNA_ligase_aden" amino acids 4 to 320 (317 residues), 380.9 bits, see alignment E=1.9e-117 TIGR00575: DNA ligase, NAD-dependent" amino acids 5 to 664 (660 residues), 833.1 bits, see alignment E=7.8e-255 PF03120: DNA_ligase_OB" amino acids 324 to 398 (75 residues), 110.5 bits, see alignment E=1.2e-35 PF03119: DNA_ligase_ZBD" amino acids 410 to 436 (27 residues), 41.3 bits, see alignment (E = 4.5e-14) PF14520: HHH_5" amino acids 450 to 504 (55 residues), 23 bits, see alignment 3.6e-08 amino acids 517 to 562 (46 residues), 31.5 bits, see alignment 8.2e-11 PF12826: HHH_2" amino acids 512 to 575 (64 residues), 93.1 bits, see alignment E=3.8e-30 PF00533: BRCT" amino acids 595 to 668 (74 residues), 52.1 bits, see alignment E=2.6e-17 PF12738: PTCB-BRCT" amino acids 610 to 660 (51 residues), 27 bits, see alignment 1.5e-09

Best Hits

Swiss-Prot: 83% identical to DNLJ_VEREI: DNA ligase (ligA) from Verminephrobacter eiseniae (strain EF01-2)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 83% identity to vei:Veis_4463)

MetaCyc: 57% identical to DNA ligase (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165KS64 at UniProt or InterPro

Protein Sequence (678 amino acids)

>Ac3H11_2314 DNA ligase (EC 6.5.1.2) (Acidovorax sp. GW101-3H11)
VALALRAQLNHWAHQYYVLDEPTVPDAEYDRVFRELQALEAAHPDLVTPDSPTQRVIGAV
MEGLAPVRHTVPMLSIHTETDTEATGAQAFDARVRRELGLADADPAIEYVAEPKFDGLAM
SLRYENGRLVQAATRGDGEVGEDVTHNIRTIRQIPLTLPSGVPPLLEVRGEVYMRRADFD
ALNERQREQGGKTFVNPRNAAAGAVRQLDSNITAQRPLSFFAYGLGAITPPAEGGPVFRT
HYEMLQTLKSWGFPVATQVQIAVDATELVAFHQQVGASRDALPYDIDGVVYKVNSLQLQR
DLGFKTREPRWAVAHKYPAQEMATKIEGIDVQVGRTGKITPVARLAPVFVGGVTVTNATL
HNLFEIRKKGVRVGDTVIVRRAGDVIPEVVGVMPGNRTGYVPNFQMPTACPVCGSAVVRE
KGEANHRCTGGLFCAAQRKEAILHFAARRAMDIEGLGDKLVDQLVDANVIRTLPDLYRLG
LTSLIALERMAEKSAQNVLAALEKSKQTTLPRFLFGLGLRHVGEATAKDLAKHFGTLDAI
MDASVEQLLEVNDVGPVVAQSLHTFFAQPHNREVVEQLRACGVTWPEGPVAERAPQILAG
KTVVLTGTLPTLSREAAKDMLEAAGAKVAGSVSKKTSYVVAGAEAGSKLAKAEELGVPVL
DEAGMLALLSGAGAGAGA