Protein Info for Ac3H11_2017 in Acidovorax sp. GW101-3H11

Annotation: UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 7 to 456 (450 residues), 546.7 bits, E=2.6e-168
• PF01225: Mur_ligase
  • amino acids 7 to 105 (99 residues), 96.6 bits, E=1.4e-31
• PF08245: Mur_ligase_M
  • amino acids 110 to 293 (184 residues), 88 bits, E=1.3e-28
• PF02875: Mur_ligase_C
  • amino acids 313 to 402 (90 residues), 57.5 bits, E=2e-19

Best Hits

Swiss-Prot: 88% identical to MURC_VEREI: UDP-N-acetylmuramate--L-alanine ligase (murC) from Verminephrobacter eiseniae (strain EF01-2)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 88% identity to vei:Veis_4571)

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See A0A165KNX7 at UniProt or InterPro

Protein Sequence (477 amino acids)

>Ac3H11_2017 UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8) (Acidovorax sp. GW101-3H11)
MKHAIRHIHFVGLGGAGMSGIAEVLFNLGYRISGSDLADSATLRRLQGLGIQTCLGHAAA
HIDGADAVVTSTAVTPDNPEVLAAREKKIPVVPRALMLAELMRLKQGIAIAGTHGKTTTT
SLVTSVLAEAGLDPTFVIGGKLNSAGANAKLGSGDYIVVEADESDASFLNLLPVMAVVTN
IDADHMETYGHDFGRLKGAFVDFLHRMPFYGTAILCVDSPAVRDILQSVTCPITSYGFSE
DAQVRAINVQAEAGQMRFTVQRRNGVTLPDMEIVLNLAGEHNVLNALSAIAVAVELNIPD
EAVQRALAGFKGVGRRFQRYGELPAKDGGQFTVIDDYGHHPVEMAATLAAARGAFPGRRL
VLAFQPHRYSRTRDCFEDFVKVIGNADAVLLTEVYAAGEAPVVAADGRSLARALRVAGRV
EPVFIDNVADLPQAIADNAKDGDVVMCMGAGSIGAVPGKVVDMLQNSELKALKGSAV