Protein Info for Ac3H11_2013 in Acidovorax sp. GW101-3H11

Annotation: UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 675 transmembrane" amino acids 496 to 517 (22 residues), see Phobius details TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase" amino acids 173 to 661 (489 residues), 339.8 bits, see alignment E=1.5e-105 PF21799: MurD-like_N" amino acids 173 to 263 (91 residues), 50.5 bits, see alignment E=2.1e-17 PF08245: Mur_ligase_M" amino acids 291 to 503 (213 residues), 102.2 bits, see alignment E=3.8e-33

Best Hits

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 74% identity to aaa:Acav_0750)

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165L4G0 at UniProt or InterPro

Protein Sequence (675 amino acids)

>Ac3H11_2013 UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) (Acidovorax sp. GW101-3H11)
MNPNDPSTIPGTSDVPEATPTEAGLGVGAPGAASAGPMPDGVALPGSSAVPATPASEADV
DAGVDTDLGPPAETAPELTAAPDAEAPAPATEAPATAPAPSTAWNPEAVPAVSAAKAAAD
FVAQIFAEVAAPEGPAAAEPDAAQDADVGEDAAPAEPLVSPVEAMAQLLQGQNILILGLG
ASGMAMARWCVRCGAQVTVADTRAAPPQLPALQQEMPQVRFVAGAFDASLVVGHNLQAVY
RSPGLSPQQVAPVLRAAEANGVRAGGELSLYSDALAALRASHAYAPAVLAITGTNGKTTV
TSLTGQLVERAGKSVAVAGNIGPTLLDTLATHLDADTLPEVWVLELSSFQLDGTNVGEGG
FEPTAATVLNITQDHLDWHGGMDAYAAAKARIFGQAGLMILNREDPAVMAMLPPPVKVKL
QKPQVRAHITFGADMPRRPGDFGIEVVSGMPWLVRAMDADETRKRGRNEVEEELHIQRLM
PADALRIRGRHNATNALAALALASATGCPLAPMLYGLREYRGEPHRVEPVAILHGVEYFD
DSKGTNVGATAAALVGLGAERRLVVILGGEGKGQDFAPLAAPVARYARAVVLIGRDAPLI
RTALQDTGVALLDAPTLPDAVALAAQRAHAGDAVLMSPACASFDMFKDYEHRAQVFCDTV
RAMADDAGQSAEGMA