Protein Info for Ac3H11_2010 in Acidovorax sp. GW101-3H11

Annotation: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 502 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 25 to 493 (469 residues), 442.9 bits, see alignment E=8e-137 PF01225: Mur_ligase" amino acids 26 to 70 (45 residues), 26.2 bits, see alignment 1.2e-09 PF08245: Mur_ligase_M" amino acids 111 to 318 (208 residues), 140.3 bits, see alignment E=1.3e-44 PF02875: Mur_ligase_C" amino acids 340 to 428 (89 residues), 98.7 bits, see alignment E=2.9e-32

Best Hits

Swiss-Prot: 74% identical to MURE_ACISJ: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Acidovorax sp. (strain JS42)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 75% identity to dia:Dtpsy_2985)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165KNS1 at UniProt or InterPro

Protein Sequence (502 amino acids)

>Ac3H11_2010 UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13) (Acidovorax sp. GW101-3H11)
MTLAVPLLTSVHDAVAWLRERVTGTLQTDSRQIRPGDGFIAWPGAATDGRAHIGDALARG
AVACLVEHEGAEAFVLSGLPVAALHGLKAATGLIAADWFEHPTQGLDVLAVTGTNGKTST
SWWLAEALNVLSKNELYGHDGCAVVGTLGMGTPPALTSTGMTTPDPVRLQRAFRQFADAG
RGACAIEASSIGLAEHRMVGTRIRVALFTNFTQDHLDYHAGMAAYWQAKSMLFDWPGLQA
AVVNIDDVRGAELHATLAGRHLDLWSVSITGPARLAARDIGMGDGGLRFTVAEGVQAHVL
QTRVIGLYNVANLLGVLAGLRALGVPLEHALYACAQLSPVPGRMEQIHQSGQPLVAVDYA
HTPDALEKALQALRPIAAERGGRLWCVFGCGGDRDAGKRPLMGAVAQQQADQVVVTSDNP
RSEDPAAILHQILQGTIASTAVQAEPDRAAAIALAVAAAAPADVVLIAGKGHENTQETAG
VHQPFSDMAHALGALQARGARP