Protein Info for Ac3H11_1931 in Acidovorax sp. GW101-3H11

Annotation: Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 16 to 210 (195 residues), 101.7 bits, E=8.8e-33
• PF16113: ECH_2
  • amino acids 59 to 214 (156 residues), 51.7 bits, E=2e-17
• PF02737: 3HCDH_N
  • amino acids 330 to 508 (179 residues), 195.1 bits, E=2e-61
• PF00725: 3HCDH
  • amino acids 511 to 611 (101 residues), 51.9 bits, E=1.8e-17

Best Hits

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 82% identity to vei:Veis_4457)

Predicted SEED Role

"Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Isobutyryl-CoA to Propionyl-CoA Module (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3)

MetaCyc Pathways

• oleate β-oxidation (29/35 steps found)
• adipate degradation (5/5 steps found)
• benzoyl-CoA degradation I (aerobic) (6/7 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• L-isoleucine degradation I (5/6 steps found)
• fatty acid salvage (5/6 steps found)
• propanoate fermentation to 2-methylbutanoate (5/6 steps found)
• pyruvate fermentation to butanol II (engineered) (5/6 steps found)
• valproate β-oxidation (7/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• adipate biosynthesis (4/5 steps found)
• L-valine degradation I (6/8 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• 3-phenylpropanoate degradation (6/10 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• phenylacetate degradation I (aerobic) (5/9 steps found)
• glycerol degradation to butanol (10/16 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (4/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• 2-methylpropene degradation (2/8 steps found)
• L-tryptophan degradation III (eukaryotic) (7/15 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
• L-glutamate degradation VII (to butanoate) (3/12 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• platensimycin biosynthesis (7/26 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• superpathway of testosterone and androsterone degradation (6/28 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3

Sequence Analysis Tools

See A0A165KLS5 at UniProt or InterPro

Protein Sequence (727 amino acids)

>Ac3H11_1931 Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) (Acidovorax sp. GW101-3H11)
MQTIRYELIPAQGTDGEVAVITFDETNSPVNTMCRQWQQELGEVTARVAADRERLGAALK
GIVLASAKTSFFAGADLKATMRLTPADAPNAFGEIERMKKHFRTLETLGIPVVACLNGAA
LGGGWEVALVAHYRIAVADPKIQFGLPEVTLGLMPGATGVTKMTRLLGLMGAQPYILEGK
LFNPAEALELQLVHELVATREQLLPAALAHISTHPHAVQPWDDKNYKMPGGTPANPKIAG
ALTVAPAMLKKTTRGRYPAPEAALAAMVEGAMVDYDTALRIESRYLARLMTGPVARNMIN
TFFFDMNAIKSGKSRPAVNGAVAPRYKPQKVGILGAGMMGAGIAWAQASRGIATVLKDVS
AEKAEAGKAYSAKLTQARVDKGRMTAEAQQALLSRITPTASAADLAGCDLIIEAVFESRD
LKAKVTQEAEPQLAPGGFFASNTSTLPISGLATASSRPEKFVGIHFFSPVDKMKLVEIIR
GNQTDDETVARAFDYVQALGKVPIVVNDSRGFYTSRTFGTFVMEGAAMLGEGIPAAAIEN
AAMQAGLPVGPLAVMDETALTLSVHVLDQTRADYATEGKTYTATPGELLVERMVKELKRP
GRAGGGGFYDYPAGAKKHLWPELKPLFEKPGVEWSVQEIQDRLLYRQAIETARCLAEGVL
TSVHDANIGSIFGIGFPAWTGGAMQFIYGTGVEAFLKRADELAAKYGTGFALTARARATV
ANFQPVY