Protein Info for Ac3H11_1829 in Acidovorax sp. GW101-3H11

Annotation: tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 412 PF01743: PolyA_pol" amino acids 1 to 122 (122 residues), 60.7 bits, see alignment E=2.7e-20 PF12627: PolyA_pol_RNAbd" amino acids 147 to 209 (63 residues), 48.8 bits, see alignment E=7.5e-17 PF01966: HD" amino acids 228 to 325 (98 residues), 37.8 bits, see alignment E=3.2e-13

Best Hits

Swiss-Prot: 83% identical to CCA_ACIET: Multifunctional CCA protein (cca) from Acidovorax ebreus (strain TPSY)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 83% identity to dia:Dtpsy_0521)

MetaCyc: 57% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.25, 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165KJ78 at UniProt or InterPro

Protein Sequence (412 amino acids)

>Ac3H11_1829 tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25) (Acidovorax sp. GW101-3H11)
MVGGAVRDKLLGRPVNDRDWVVVGATPEQMLALGYLPVGRDFPVFLHPETREEYALARTE
RKSGRGYRGFVVHSAPDVTLEEDLSRRDLTINAIAENTDGTSAEGIFDPYSGAQDIEARV
LRHVTDAFREDPVRILRVARFAARFTDFSVAPETMQLMREMVEHGEVDHLVPERVWQELA
RGLMEEQPSRMFEVLRDCGALAVLLPEVARLWGVPQRAEYHPEVDTGVHLMMVLDMAARL
SAPLTVRFACLAHDLGKGTTPADVLPRHIDHETRSAELLKDVAERLRVPVDCRETADVVA
REHGHIHRSTDLSAAALVRLLERCDAIRKPGRFAEILLACECDARGRLGFEESAYPQRPR
LLAVLGAVQSVVTREIAAQAAAKGLSGPQVGGLIHQARVAAVAQWLKAPTAL