Protein Info for Ac3H11_153 in Acidovorax sp. GW101-3H11

Annotation: Similar to 5-oxoprolinase (EC 3.5.2.9) and Methylhydantoinases A, B (EC 3.5.2.14), contradiction in experimental data (see Annotation)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1214 PF05378: Hydant_A_N" amino acids 12 to 190 (179 residues), 176.4 bits, see alignment E=9.1e-56 PF01968: Hydantoinase_A" amino acids 210 to 490 (281 residues), 361 bits, see alignment E=9e-112 PF19278: Hydant_A_C" amino acids 511 to 667 (157 residues), 48.3 bits, see alignment E=1.6e-16 PF02538: Hydantoinase_B" amino acids 690 to 1210 (521 residues), 657 bits, see alignment E=3.8e-201

Best Hits

KEGG orthology group: K01469, 5-oxoprolinase (ATP-hydrolysing) [EC: 3.5.2.9] (inferred from 76% identity to ajs:Ajs_2480)

Predicted SEED Role

"Similar to 5-oxoprolinase (EC 3.5.2.9) and Methylhydantoinases A, B (EC 3.5.2.14), contradiction in experimental data (see Annotation)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 3.5.2.14, EC 3.5.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.2.14, 3.5.2.9

Use Curated BLAST to search for 3.5.2.14 or 3.5.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165IXL6 at UniProt or InterPro

Protein Sequence (1214 amino acids)

>Ac3H11_153 Similar to 5-oxoprolinase (EC 3.5.2.9) and Methylhydantoinases A, B (EC 3.5.2.14), contradiction in experimental data (see Annotation) (Acidovorax sp. GW101-3H11)
MTTAPAGNRWQFWIDRGGTFTDIVAKRPDGSLTTHKLLSENPEQYKDAAVAGIRHLLGLQ
PGEPVTPALVECVKMGTTVATNALLERKGEPTLLVTTKGFKDALRIAYQNRPRLFDRHIQ
LPELLYTEVIEARERMGARGNLLQNLDEATLRGDLLAAYGRGLRSVAIVFMHGYRYTQHE
QAAKRIAKEVGFTQISTSHETSPMMKFVSRGDTTVVDAYLSPILRRYVEQVASEMPGVKL
FFMQSSGGLTDAGTFQGKDAILSGPAGGIVGMARTAGLAGHEKVIGFDMGGTSTDVSHYA
GAFEREFETHVAGVRMRAPMMSIHTVAAGGGSVLAYDGSRFRVGPESAGANPGPVSYRRG
GPLAVTDANVMVGKVQPRYFPSVFGPAANETLDADAVRARFQEIATQTQRQPEEVAEGFI
QIAVQQMANAIKKISVARGYDVTRYTLQCFGGAGGQHACLVADALGMSRVFVHPLAGVLS
AYGMGLADQTVIREQAVEMPLAAASLPLIAERLDTLGSAAQAELERQQVSANPVQVRHNV
HVRYEGTDSALVVPFGDITSIQAAFEKAYRQRFAFLMAGKGLVVEAVSVEAMIAGDAPAE
PRLPVHPHRKHPQRESVRMYSGGEWHTAALVVREDLHPGDVIPGPAIIAEKNTTTVVEPG
WSARLTDLDHLVLDRVTARKVQYAAGTTVDPVLLEVFNNLFMNIAEQMGLQLQNTAYSVN
IKERLDFSCALFDAAGNLIANAPHMPVHLGSMGESIKTVIRENAGKMHPGDVYMLNDPYH
GGTHLPDVTVITPVYVAEGAEPTFYVGSRGHHADIGGTTPGSMPPFSTRIDEEGVQINNV
KLVDRGIFLEDSVRNLLATGGGTTPYPSRNPEQNLADLRAQIAANEKGVQELSKMVEQFG
LDVVQAYMCHVQDNAEESVRRVITQLKDGQFTLPLDNGAQISVSVKVNVAERSAVIDFAG
TSPQQMNNFNAPRAVCMAAVLYVFRTLVDDDIPLNAGCLKPLQVIIPQGSMLNPNPPASV
VAGNVETSTCITNALFGALGVMAGSQPTMNNFTFGNAQYQYYETIAGGSGAGAVFDASGR
TVRGFDGTSVVQTHMTNSRLTDPEVLEFRFPVVLDSYEIQRGSGGAGRWAGGDGGVRRVR
FLEAMTASILSNGRLNPAFGMAGGQPGRAGANRVLRANGQVEELGHIGAALMEPGDVFEI
STPGGGGFGECVAH