Protein Info for Ac3H11_148 in Acidovorax sp. GW101-3H11

Annotation: 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 PF00106: adh_short" amino acids 11 to 211 (201 residues), 188.6 bits, see alignment E=1.8e-59 PF01370: Epimerase" amino acids 13 to 193 (181 residues), 26.9 bits, see alignment E=6.2e-10 PF08659: KR" amino acids 13 to 126 (114 residues), 52.4 bits, see alignment E=1.3e-17 PF13561: adh_short_C2" amino acids 16 to 258 (243 residues), 196.5 bits, see alignment E=1.1e-61

Best Hits

Swiss-Prot: 36% identical to DTHD_MYCS2: D-threitol dehydrogenase (dthD) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: K00540, [EC: 1.-.-.-] (inferred from 93% identity to vei:Veis_3982)

MetaCyc: 36% identical to D-threitol dehydrogenase (Mycolicibacterium smegmatis)
ERYTHRULOSE-REDUCTASE-RXN [EC: 1.1.1.403]

Predicted SEED Role

"3-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 1.1.1.30

Use Curated BLAST to search for 1.-.-.- or 1.1.1.30 or 1.1.1.403

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165IXJ7 at UniProt or InterPro

Protein Sequence (260 amino acids)

>Ac3H11_148 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) (Acidovorax sp. GW101-3H11)
MAYSIDLSGRVAFITGASSGLGAQFARTLARAGAGVVLASRRIEKLKELRARIEGEGGDA
HVIELDVTDHASIKSAVAHAETEMGSIDILINNSGVSTTQRLQDVTPEDYDFIFDTNVKG
AFFVAQEVGKRMLARSRGAAPGSFTGGRIINIASMAGLKVLPQIGAYCMSKAAVVQMTRA
MAMEWGKFGINTNAICPGYIDTEINHHHWDTEQGQKLIAMLPRKRVGSPQDLDALLVMLA
SDQSHFINGAVIAADDGFAV