Protein Info for AZOBR_RS33830 in Azospirillum brasilense Sp245

Annotation: mannose-1-phosphate guanylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 10 to 477 (468 residues), 736 bits, E=8.8e-226
• PF00483: NTP_transferase
  • amino acids 12 to 294 (283 residues), 217.9 bits, E=3.4e-68
• PF01050: MannoseP_isomer
  • amino acids 324 to 473 (150 residues), 249.4 bits, E=2.5e-78
• PF07883: Cupin_2
  • amino acids 390 to 457 (68 residues), 44.9 bits, E=1.5e-15

Best Hits

Swiss-Prot: 63% identical to XANB_XANCP: Xanthan biosynthesis protein XanB (xanB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 72% identity to rru:Rru_B0046)

MetaCyc: 55% identical to phosphomannose isomerase / GDP-D-mannose pyrophosphorylase (Pseudomonas aeruginosa)
Mannose-6-phosphate isomerase. [EC: 5.3.1.8]; Mannose-1-phosphate guanylyltransferase. [EC: 5.3.1.8, 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• GDP-mannose biosynthesis (4/4 steps found)
• D-mannose degradation II (2/2 steps found)
• mannitol degradation II (3/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (9/14 steps found)
• mannitol biosynthesis (1/3 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22 or 5.3.1.8

Sequence Analysis Tools

See G8B1G8 at UniProt or InterPro

Protein Sequence (481 amino acids)

>AZOBR_RS33830 mannose-1-phosphate guanylyltransferase (Azospirillum brasilense Sp245)
MGSKASASTVTPVILSGGAGSRLWPLSRASYPKQFLPLASEQTMIQETALRVSSDRFAAP
LVICNEEHRFIVAEQLRAASVSPAEIILEPVGRNTAPAVCIAALALLESGEDRLMLVMPS
DHVIARSDRFLEAVEQAAVAAAAGSLVTFGITPVAPETGYGYIKAGGPLGTGDNIRVVER
FVEKPDLATAQTYLADGRYLWNSGIFLFSAAAYVAELEKSNPAIVEACRQARSSAARDLT
FCRLEKEAFAASPSDSVDYAVMEKTDQAAVVAVDMGWNDVGAWSALWDIAEKDESGNVVH
GDVVLHNARNSYVRSEEQLVAVAGLDDVVVIATDDAVLVADRKHVQDVKLIVERLKAEKR
DEHALHTTVYRPWGSYRGIDIGDRFQVKRITVKPGERLSLQMHHHRAEHWIVVQGTALVT
CGEKQELLFENQSVYIPMGTTHRLENPGKVPLHLIEVQSGSYLGEDDIVRFEDGYGRVAG
K