Protein Info for AZOBR_RS33695 in Azospirillum brasilense Sp245

Annotation: 4-hydroxy-4-methyl-2-oxoglutarate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 43% identical to GALC_PSEPK: 4-carboxy-4-hydroxy-2-oxoadipic acid aldolase (galC) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K10218, 4-hydroxy-4-methyl-2-oxoglutarate aldolase [EC: 4.1.3.17] (inferred from 70% identity to azl:AZL_004930)

MetaCyc: 43% identical to subunit of 4-hydroxy-4-methyl-2-oxoglutarate aldolase (Pseudomonas putida)
4-hydroxy-4-methyl-2-oxoglutarate aldolase. [EC: 4.1.3.17]; 4.1.3.17 [EC: 4.1.3.17]

Predicted SEED Role

No annotation

MetaCyc Pathways

protocatechuate degradation I (meta-cleavage pathway) (8/8 steps found)
superpathway of vanillin and vanillate degradation (9/10 steps found)
methylgallate degradation (6/6 steps found)
gallate degradation II (4/5 steps found)
gallate degradation I (3/4 steps found)
4-hydroxy-4-methyl-L-glutamate biosynthesis (1/2 steps found)
syringate degradation (8/12 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.17

Use Curated BLAST to search for 4.1.3.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8B1E0 at UniProt or InterPro

Protein Sequence (222 amino acids)

>AZOBR_RS33695 4-hydroxy-4-methyl-2-oxoglutarate aldolase (Azospirillum brasilense Sp245)
MTVVKEFPRPAPDLVAAFRGQSPATLHEAMGKKGAMSFPIKPLYQGMTLFGTAVTVSGGP
TDNLMIHAAMALTRPGDVLVVDFKGMTEAGPWGDVLTEAAIQRGLAGLVIDGCVRDAAAI
RNMGFPVFCRGTSMKGTTKTQPTGDVNTPIVCGGVMVMPGDILVGDDDGVVVVPQAEAAA
TLAKAAERERMEEGLREQLRAGRTTVQALTLEPYLEAGGIAL