Protein Info for AZOBR_RS33565 in Azospirillum brasilense Sp245

Annotation: UDP-N-acetyl-D-galactosamine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03721: UDPG_MGDP_dh_N
  • amino acids 6 to 178 (173 residues), 116.9 bits, E=1.7e-37
• TIGR03026: nucleotide sugar dehydrogenase
  • amino acids 6 to 405 (400 residues), 382.5 bits, E=1.1e-118
• PF00984: UDPG_MGDP_dh
  • amino acids 201 to 289 (89 residues), 90.3 bits, E=1.5e-29
• PF03720: UDPG_MGDP_dh_C
  • amino acids 318 to 418 (101 residues), 78.9 bits, E=6.8e-26

Best Hits

Swiss-Prot: 57% identical to CAPL_STAAU: Protein CapL (capL) from Staphylococcus aureus

KEGG orthology group: K02474, UDP-N-acetyl-D-galactosamine dehydrogenase [EC: 1.1.1.-] (inferred from 66% identity to bra:BRADO5154)

MetaCyc: 60% identical to UDP-N-acetylgalactosamine 6-dehydrogenase (Pseudomonas aeruginosa)
UDP-N-acetylglucosamine 6-dehydrogenase. [EC: 1.1.1.136]; 1.1.1.136 [EC: 1.1.1.136]

Predicted SEED Role

"UDP-glucose dehydrogenase (EC 1.1.1.22)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) or Teichuronic acid biosynthesis (EC 1.1.1.22)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (11/11 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (5/6 steps found)
• UDP-α-D-xylose biosynthesis (2/2 steps found)
• UDP-α-D-glucuronate biosynthesis (from UDP-glucose) (1/1 steps found)
• UDP-N-acetyl-α-D-galactosaminuronate biosynthesis (1/2 steps found)
• UDP-sugars interconversion (4/9 steps found)
• UDP-2,3-diacetamido-2,3-dideoxy-α-D-mannuronate biosynthesis (1/5 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of type II polyketide products
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Butanoate metabolism
• C21-Steroid hormone metabolism
• Fructose and mannose metabolism
• Glycine, serine and threonine metabolism
• Insect hormone biosynthesis
• Linoleic acid metabolism
• Nucleotide sugars metabolism
• Pentose and glucuronate interconversions
• Polyketide sugar unit biosynthesis
• Retinol metabolism
• Starch and sucrose metabolism
• Tetrachloroethene degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.22

Use Curated BLAST to search for 1.1.1.- or 1.1.1.136 or 1.1.1.22

Sequence Analysis Tools

See G8B1B2 at UniProt or InterPro

Protein Sequence (430 amino acids)

>AZOBR_RS33565 UDP-N-acetyl-D-galactosamine dehydrogenase (Azospirillum brasilense Sp245)
MTHSRRISVIGLGYVGLPVAVAFGRMDVPVVAFDIDARRIAALRAGHDHTGEVADTDLAA
ARLHLTDDPADLAQADFHIVTVPTPIDDARRPDLRPLLAASRTVGRHLKRGDVVVYESTV
YPGATEMDCVPVLEAESGLTFGRDFTIGYSPERINPGDKEHRFETITKVVSGSDPATRSL
VAAVYGSVVKAGVHEAASLAVAEAAKVIENTQRDVNIALMNELAVIFHRMGIDTRDVLAA
AGTKWNFLKFQPGLVGGHCIGVDPYYLTHRAEQLGHNPEVILAGRRINDTMGQYVAQETV
KRLLRGRVGASGPLRVTVLGLTFKEDVPDIRNTRVVDIVAELKSFGVEVALHDPLAAADE
VAHEYGLTLTARDALPPADAVVLAVPHAAYRTEGWDLVSGLLKDRRGIVMDVKGLLDRGA
VPEGVDLWRL