Protein Info for AZOBR_RS30685 in Azospirillum brasilense Sp245

Annotation: DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 955 PF02739: 5_3_exonuc_N" amino acids 26 to 187 (162 residues), 189.8 bits, see alignment E=6e-60 TIGR00593: DNA polymerase I" amino acids 26 to 955 (930 residues), 931.2 bits, see alignment E=3.9e-284 PF01367: 5_3_exonuc" amino acids 188 to 283 (96 residues), 99.2 bits, see alignment E=3.2e-32 PF01612: DNA_pol_A_exo1" amino acids 344 to 542 (199 residues), 115.7 bits, see alignment E=4.1e-37 PF00476: DNA_pol_A" amino acids 575 to 953 (379 residues), 511.7 bits, see alignment E=2.4e-157

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 70% identity to rce:RC1_2483)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AZG2 at UniProt or InterPro

Protein Sequence (955 amino acids)

>AZOBR_RS30685 DNA polymerase (Azospirillum brasilense Sp245)
MSDEKTTDTLQTATDAPAPAGDGSGLYLVDGSGFIFRAFHALPMLNRPDGTPVNAVLGFS
NMLLKLLADLKASAVAVVFDSKRLNFRNEFYPEYKAHRPEPPEELKPQFALIREATEAFC
LPCLELEGYEADDLIATYARLANEAGREVTIVSSDKDMMQLVRPGVRMLDPLKNKPIGPD
EVFEKFGVAPDRVVDVQALAGDSVDNVPGVPGIGVKTAAQLITEYGDLESLLARAGEIKQ
PARRQKLIDFAEQARISRRLVLLDDHAPPPKPLEELRVREPDHQKLIDFLRAQGFRTIVS
RVEAEMRKDGTIADGAVPSSATPAAEVGAPRSRPAPPTDVEQRYELVQDLDALAVWLERA
RTTGVLAVDTETNSLTPATATLVGISLATEPGLACYIPLAHQAPNAAASGELSFETPEAP
KQIPAEDALKALKEVLEDPAILKIGHNFKFDHQLFARHGVRVSPVDDSMLISYVLEGGAH
GHGMDELAELHLAHTTIPYKEVCGTGKNQITFDRVPLDKALAYAAEDADITLRLWRALKP
RLVDERMVTVYETLERPLVPVIADMESCGIRVDRAALARLSQDLAVRMAEIEKEVHQHAG
RSFNIGSPKQLGEILFDEMKLGTGKKGKTGAYSTDSSVLEELAEQGHVIAQRVLDWRQLA
KLKNTYTDALQAQIVEGSERVHTAFAMAATNTGRLSSTDPNLQNIPVRTEEGRKIRRAFV
AAPGHKLISIDYSQIELRLVAEMADIAALKQAFQDGIDIHAATASQVFGVPLDQMTSEIR
RKAKAINFGIIYGISGFGLGRQLGIAPGEANAFIKAYFERFHELKLWMEATKAFARQNGH
VVTLFGRRCYIPGIHDKNAARRAFAERQAINAPIQGTAADIMKRAMARVPGALKEAGFDE
VRMLLQVHDELLFEAPEDQAERAAALVRQVMEGAATLGVPLVAEAGIGGNWDEAH