Protein Info for AZOBR_RS30030 in Azospirillum brasilense Sp245

Annotation: twin-arginine translocation pathway signal protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729 PF20256: MoCoBD_2" amino acids 52 to 200 (149 residues), 41.9 bits, see alignment E=9.5e-15 amino acids 582 to 678 (97 residues), 68.3 bits, see alignment E=8.5e-23 PF02738: MoCoBD_1" amino acids 314 to 556 (243 residues), 115.4 bits, see alignment E=2.4e-37

Best Hits

KEGG orthology group: K07303, isoquinoline 1-oxidoreductase, beta subunit [EC: 1.3.99.16] (inferred from 77% identity to azl:AZL_e03260)

Predicted SEED Role

"Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16)" in subsystem N-heterocyclic aromatic compound degradation (EC 1.3.99.16)

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.16

Use Curated BLAST to search for 1.3.99.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AZ09 at UniProt or InterPro

Protein Sequence (729 amino acids)

>AZOBR_RS30030 twin-arginine translocation pathway signal protein (Azospirillum brasilense Sp245)
IKQAAADVAAPSRRGFLKGAGGALVLGAMLPRTGFASEAAAPKVVGPADPRPQAFIRIAA
DDTVTVLVKHLDKGQGIMTGLTTIVAEELDADWAQMRGAFAPADGALYANHFFGIQGTGG
STAVANSWDELRMAGAAARAMLVATAAARWSVPASPVTVEQGVVRHAASNRSARFGELAE
DAAKLPVPQNVALKDAKDYKLIGNPSLHRLDHVSKTDGTAIFAMDIRRPGQVTAVLARSP
RFGGTVKSVDAAAAKQVPGVLEVLTLPMGVAVVARNTWAAMKGREALTVEWDDAKAEMRG
TEEMLADYRKLADQPGNVATDTGDAAKALAGAAKVVEAEFVFPYLAHAPMEPLNATVELT
PDGGCTIWAGSQFQGVEQKVAAGILGCKPEQVVINTVWAGGSFGRRATPDADYIAEAVTI
AKAYGGKAPVHLVWTREDDIRGGRYRPLFLHKVKAGLDASGTLVAWRQSIVGQSFMIGTL
FEGFMVKNGVDATSVEGSSDMAYTVPNLHVDLHSPPSPVTTLWWRSVGHTHTAYAKEVMI
DELAAAAGKDPVAFRLEMLKDHPRLQGVLKLAAEKAGWGQPLPKGKGRGIAVHESFSSFV
AHVAEVSVDEQGRVTIDRVVCAVDCGQVVNPNIVEAQVSGGTGWAIGHALRDEITLTGGL
VDQSNFDAYQPLRNSEMPPIEVHILPSTARPTGVGEPGVPTAAPAVANAVFAATGKRLHH
LPFTRSGMV