Protein Info for AZOBR_RS26030 in Azospirillum brasilense Sp245

Annotation: beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 PF00232: Glyco_hydro_1" amino acids 5 to 438 (434 residues), 476.3 bits, see alignment E=4e-147 TIGR03356: beta-galactosidase" amino acids 6 to 431 (426 residues), 578.2 bits, see alignment E=5.2e-178

Best Hits

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 81% identity to azl:AZL_e01410)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21, 3.2.1.23

Use Curated BLAST to search for 3.2.1.21 or 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AWC9 at UniProt or InterPro

Protein Sequence (444 amino acids)

>AZOBR_RS26030 beta-glucosidase (Azospirillum brasilense Sp245)
MALFTFPKDFLWGASTAAYQIEGAIHEDGRGPCVWDTFTEAGRIQDGSSAAVACDHYHRW
AEDTALMKDAGFNAYRFSIAWPRILPSGTGAVNAAGLDFYDRLVDGLLADGIRPMACLYH
WDLPQPLEDRGGWQGREVVGPFADYARIVAARLADRVKDWMMLNEPNVVATHGYGTGEHA
PGHKLGETGILRALHHQNLAQGAALRALSAEHSGLTLGTVINLQPCRAESDRPQDVAAAA
RWDAVWNRVALDGVMRGKIPELMAERMKDFVLPGDEEAIRFPIDLLGINYYSRMTMKHET
GHPFDVWWGDAHCDRWTAMAWPVQPDGLYDLLMEFRRDYGNPAVFVAENGAAYDDVVTPD
GQVHDAERVAFLRDHVASVGRALADGCNVKGYLCWSLLDNFEWAFGLSKRFGIVRVDYDT
LVRTPKDSYRFLSEVAKSGQFDLA