Protein Info for AZOBR_RS25975 in Azospirillum brasilense Sp245

Annotation: chemotaxis protein CheR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 PF03705: CheR_N" amino acids 21 to 70 (50 residues), 53.7 bits, see alignment 1.3e-18 PF01739: CheR" amino acids 88 to 275 (188 residues), 187.8 bits, see alignment E=1.5e-59

Best Hits

Swiss-Prot: 48% identical to CHER2_VIBCH: Chemotaxis protein methyltransferase 2 (cheR2) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00575, chemotaxis protein methyltransferase CheR [EC: 2.1.1.80] (inferred from 76% identity to azl:AZL_d03460)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80

Use Curated BLAST to search for 2.1.1.80

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AWB5 at UniProt or InterPro

Protein Sequence (291 amino acids)

>AZOBR_RS25975 chemotaxis protein CheR (Azospirillum brasilense Sp245)
MSLPSDKGPSDKGDTAGLSAADYDAFCRFLRERTGLSFTEAKRYFVDRRVSERMAAVGVE
HFRQYLNLLRYQASGEELQLLVNRMTVNETYFFREMYQLDCLVNAALDEVVSGREPGSRV
RIWSAGCATGEEPYSIAITLLERWSRVDEFEIELYASDIDSHVLERAQEGIYEERSLQAM
PRDLVAKYFTRLDDQRWQIMEELRDSIDFSLVNIADPRQMANFRGMDVIFCRNLLIYFDD
LGRREAASMFYDALSPCGFICLGHSESMSRMSSLFVPRRFPDAILYQKPQS