Protein Info for AZOBR_RS25660 in Azospirillum brasilense Sp245

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 TIGR01891: amidohydrolase" amino acids 15 to 374 (360 residues), 370.6 bits, see alignment E=4.4e-115 PF01546: Peptidase_M20" amino acids 72 to 383 (312 residues), 170 bits, see alignment E=6.5e-54 PF07687: M20_dimer" amino acids 186 to 278 (93 residues), 25.4 bits, see alignment E=1.2e-09

Best Hits

Swiss-Prot: 37% identical to Y100_SYNY3: Uncharacterized hydrolase sll0100 (sll0100) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 63% identity to scl:sce2384)

MetaCyc: 48% identical to beta-tabtoxin peptidase (Pseudomonas syringae)
3.4.11.-

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8AW41 at UniProt or InterPro

Protein Sequence (390 amino acids)

>AZOBR_RS25660 amidohydrolase (Azospirillum brasilense Sp245)
MVRTIDGTEALTADMTAWRRDLHAHPETAFEEERTSEFVAEKLASFGLEVHRGLAKTGVV
GVLRNGEGPAVAFRADMDALHIHEQTNLPHASRNPGRMHACGHDGHTAMLLGAARHLSAH
PNFQGTIAFVFQPAEENEGGGRVMVEDGLFEKFPVEQVYGMHNWPGLEVGKIALRPGPIM
AAYDIFELTLTGKGTHAAMPHLGTDTILAGTQIVNAWQTIASRSVHPVDSAVVSVTQFHA
GDTWNVLPATAVLRGTTRTFRKEVQDMVERRMGELAKAIASGFGVEAEMRYERRYPSTVN
EAGATELARRAAAGVVGESGLDLDPMPSMGAEDFAFMLQQRPGCYVWVGAGPSDGGRNLH
SPHYDFNDAVLPIGLSYWVRLAETVLPRAA