Protein Info for AZOBR_RS25530 in Azospirillum brasilense Sp245

Annotation: sugar aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01380: SIS
  • amino acids 46 to 162 (117 residues), 43.7 bits, E=1.2e-15
  • amino acids 205 to 284 (80 residues), 53.1 bits, E=1.5e-18

Best Hits

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 57% identity to msv:Mesil_0290)

Predicted SEED Role

"Glucosamine-6-phosphate deaminase [isomerizing], alternative (EC 3.5.99.6)" in subsystem Chitin and N-acetylglucosamine utilization or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 3.5.99.6)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• N-acetylglucosamine degradation II (3/3 steps found)
• N-acetylglucosamine degradation I (2/2 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• chitin derivatives degradation (4/8 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation (2/6 steps found)
• chitin degradation I (archaea) (1/7 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16 or 3.5.99.6

Sequence Analysis Tools

See G8AW10 at UniProt or InterPro

Protein Sequence (346 amino acids)

>AZOBR_RS25530 sugar aminotransferase (Azospirillum brasilense Sp245)
MTSAPLMMQEAVTAPAAVRRLLDSDTAAVAALADRLRAVPPPFAMTIARGSSDHAAGYAR
YLFETALGLVTASAAPSVVTAYGADLRVKDAFVLAISQSGQSPDLLCVAEAARAGGAVTA
ALVNAEDAPLAERVAHPLPLHAGPELSVAATKSFVASLAAIARLTAVWMDDRTLLDALPR
LPFWLERAGAADWSAALPVLEAVSSLLIVARGRSYPIAQEMALKFKETAAAHAEPFSAAE
VMHGPMALVEPGFPVLVVAVRDETLDGVLDTARALKEAGAHLLVASAEERALALADTPLL
LPPPLHPVLDPIAAIQAFYPFMARLAVARGFDPDRPRHLRKVTRTI