Protein Info for AZOBR_RS25430 in Azospirillum brasilense Sp245

Annotation: Citrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 10 to 222 (213 residues), 146.3 bits, E=8.9e-47
• PF15617: C-C_Bond_Lyase
  • amino acids 12 to 252 (241 residues), 58.6 bits, E=6.1e-20

Best Hits

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 41% identity to ypi:YpsIP31758_2156)

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (6/6 steps found)
• citrate degradation (2/2 steps found)
• mevalonate degradation (1/2 steps found)
• ketogenesis (3/5 steps found)
• reductive TCA cycle II (7/12 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Citrate cycle (TCA cycle)
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.34, 4.1.3.4, 4.1.3.6

Use Curated BLAST to search for 4.1.3.34 or 4.1.3.4 or 4.1.3.6

Sequence Analysis Tools

See G8AVZ0 at UniProt or InterPro

Protein Sequence (283 amino acids)

>AZOBR_RS25430 Citrate lyase (Azospirillum brasilense Sp245)
MTDLRSVRRRSFLFVPGLRPDRFAKALETGADVVCIDLEDAVAPDRKDEARALSLPTYHE
QRAARAEKALRINSIRSPEGIADVDAILKLETLPDALVLPKVRSADEVRIVADLLRSRPE
PVALYVIIETAEGLERVAEIAEADPSIVALFIGAVDLSAELRVRPTWDALLYARSRIVHA
AARAGVAVLDVPFLDLEDAAGLETEARRAAALGFTGKALIHPGHLPAIHAAFTPTEEEVA
HARRIIAAFQESTTGLVVVDGKLIEKPVIREMERILFTAGTAA