Protein Info for AZOBR_RS24500 in Azospirillum brasilense Sp245

Annotation: N(5) N(10)-methenyltetrahydromethanopterin cyclohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03120: methenyltetrahydromethanopterin cyclohydrolase
  • amino acids 12 to 325 (314 residues), 382.6 bits, E=7e-119
• PF02289: MCH
  • amino acids 12 to 325 (314 residues), 402.6 bits, E=6.3e-125

Best Hits

Swiss-Prot: 61% identical to MCH_METS4: Methenyltetrahydromethanopterin cyclohydrolase (mch) from Methylobacterium sp. (strain 4-46)

KEGG orthology group: K01499, methenyltetrahydromethanopterin cyclohydrolase [EC: 3.5.4.27] (inferred from 64% identity to sno:Snov_0750)

MetaCyc: 57% identical to methenyltetrahydropterin cyclohydrolase subunit (Methylorubrum extorquens AM1)
Methenyltetrahydromethanopterin cyclohydrolase. [EC: 3.5.4.27]

Predicted SEED Role

"N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27)" in subsystem Methanogenesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 3.5.4.27)

MetaCyc Pathways

• formaldehyde oxidation VI (H₄MPT pathway) (4/4 steps found)
• methyl-coenzyme M oxidation to CO₂ II (4/6 steps found)
• superpathway of C1 compounds oxidation to CO₂ (8/12 steps found)
• methanogenesis from H₂ and CO₂ (3/6 steps found)
• methyl-coenzyme M oxidation to CO₂ I (3/6 steps found)
• reductive acetyl coenzyme A pathway II (autotrophic methanogens) (3/6 steps found)
• methoxylated aromatic compound degradation II (4/9 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• superpathway of methanogenesis (5/21 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Methane metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.27

Sequence Analysis Tools

See G8AVC7 at UniProt or InterPro

Protein Sequence (329 amino acids)

>AZOBR_RS24500 N(5) N(10)-methenyltetrahydromethanopterin cyclohydrolase (Azospirillum brasilense Sp245)
MAKPNTHDSRPSLAALSAPLVERLMADAPMLRLGIRRVGGACVVDAGIDHPGGLEAGRRI
AELCMGGLGRVAFGPISSPPSWPFGVTVHSAQPVLACLGSQYAGWSLSHGSGKGAFFALG
SGPGRALARQEALFDELDYRDTADAAAFVMEATTVPPAELIAHIAATCGIVADRLTLVLT
PTQSLAGTTQVVARVLEVALHKLHALGFPLDRVVDGIGMAPLPPPGGGFLTAMGRTNDAI
LYGGTVHLHVTGPDDAAEDLAHRLPSAASRDHGRPFAEIFAAAKGDFYAIDSMLFSPARV
TVTALDSGRSFHGGTLAPDLVERSFRDVR