Protein Info for AZOBR_RS22535 in Azospirillum brasilense Sp245

Annotation: asparagine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03104: asparagine synthase family amidotransferase
  • amino acids 1 to 589 (589 residues), 1022.8 bits, E=0
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 520 (519 residues), 449.7 bits, E=1.7e-138
• PF13522: GATase_6
  • amino acids 32 to 160 (129 residues), 140.1 bits, E=6.3e-45
• PF13537: GATase_7
  • amino acids 47 to 167 (121 residues), 149.4 bits, E=7e-48
• PF00733: Asn_synthase
  • amino acids 245 to 587 (343 residues), 320.3 bits, E=3.7e-99

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 74% identity to mno:Mnod_0142)

MetaCyc: 63% identical to N-acetylglutaminylglutamine amide synthase (Sinorhizobium meliloti 1021)
RXN-13456

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (8/8 steps found)
• superpathway of L-citrulline metabolism (10/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• N-acetylglutaminylglutamine amide biosynthesis (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Sequence Analysis Tools

See G8ATZ7 at UniProt or InterPro

Protein Sequence (591 amino acids)

>AZOBR_RS22535 asparagine synthase (Azospirillum brasilense Sp245)
MCGISGEIRFDSRPASASAVGSMTEALALRGPDGQGVFAHGRMAFGHRRLSIIDLSEAAQ
QPMTDPELGLGIVFNGCIYNYPELRAELEAKGYRFFSHGDTEVLLKAYHAWGRRFVERLY
GMFAFAIWERDSGRVTLGRDRLGIKPLYLSETAGRLRFASTLPALLAGGDIDTTIDPVGL
YQYMSFHAVVPAPGTILKGVRKLPPATLLTLEPDGRRIEETYWEPVFGPPPGDERMSEGD
WQDAVLSTLRKAVERRLVADVPVGVLLSGGLDSSVIVALLAEAGQTGLQTFSIGFETVGQ
EKGDEFQYSDLIAQRFQTDHHKLFIDSNRALPALQDCVRAMSEPMVSHDAIGFFLLSQEV
AKHVKVVQSGQGADEIFAGYHWYPPMMEAADPLSTYAKVFFDRNRAEMAEALNPQYLADE
DAASAFVAAHFARPGADRPVDKALRLDTTVMLVDDPVKRVDNMTMAWGLEGRVPFLDHDL
VELAARVPPELKVRDGGKYVLKEAARKVVPSEVIDRPKGYFPVPALKYLRGPYLELVRDV
LNQPAARKRALFQPAYLDRLFSDPETHITPLRGSKLWQAALLEFWLQTHGI