Protein Info for AZOBR_RS22005 in Azospirillum brasilense Sp245

Annotation: Lon protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 810 PF02190: LON_substr_bdg" amino acids 35 to 226 (192 residues), 163.4 bits, see alignment E=1.7e-51 TIGR00763: endopeptidase La" amino acids 36 to 795 (760 residues), 911.2 bits, see alignment E=3.6e-278 PF00004: AAA" amino acids 377 to 516 (140 residues), 84.3 bits, see alignment E=2.6e-27 PF07728: AAA_5" amino acids 377 to 511 (135 residues), 31.3 bits, see alignment E=4.7e-11 PF05362: Lon_C" amino acids 595 to 797 (203 residues), 274.8 bits, see alignment E=1e-85 PF13541: ChlI" amino acids 639 to 765 (127 residues), 27.5 bits, see alignment E=6.5e-10

Best Hits

Swiss-Prot: 66% identical to LON_METSB: Lon protease (lon) from Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 66% identity to bbt:BBta_7794)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8APV8 at UniProt or InterPro

Protein Sequence (810 amino acids)

>AZOBR_RS22005 Lon protease (Azospirillum brasilense Sp245)
MKDVRMTDEHAAEATPAPQTPPASPPASGLPEDVIPVIPVRNLVQFPGVVLPVTVGRARS
VAAAQEAARTERPVGILLQRDEAVEDPTGADMHRVGTTASILRYVTTPDGSHHLVCQGQQ
RFRIVEWVPGHPFMAARVEMVEESDTATPEVMARFLNLRNEAVEALQLLPQAPQELLAAV
QALESPGELADMTASYMDLKPAEKQEVLETIDLPERLDKVGGLLARRIEVLRLSRDLRER
TREAMDERQREYLLREQLRAIQKELGEADEGRQAEIGELQDAIAKAGMPLDVREHAEKEL
RRLERMPEAAAEYSMVRSYLDWLIEMPWDRRSESRIDIAEARRILDEDHYGLEKVKRRIL
EFLAVRKLNPEGRSPILCFVGPPGVGKTSLGQSIARATGRAFARVSLGGVHDESEIRGHR
RTYVGALPGNIVQAIRKAGTRDCVLMLDEMDKLGQGFHGDPSAALLEVLDPEQNATFRDH
YLGVPFDLSKVMFIATANMLDTIPGPLRDRMEVIELSGYTEDEKLEIAKRYLLARQLAAN
GLTADQLEIPDDTLRAIIRDHTREAGNRQLERLIGAVGRYAAVRIAEGEVERMRVEPADL
TAILGPPRFENDVAMRTSVPGVATGLAWTPVGGDILFIEASRFSGSGRLILTGQLGEVMK
ESAQAALSLVKSRVKDLGLDPEGLDRFDIHIHVPAGAIPKDGPSAGVAIFTALVSLLSGR
CIRSDTAMTGEISLRGLVLPVGGIREKVVAAQRAGLKTVMLPARNRKDFDDIPAAVRERL
SFVWLERVDDAVRAALVSEPSVEAAERKAG