Protein Info for AZOBR_RS20750 in Azospirillum brasilense Sp245

Annotation: cytochrome B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 transmembrane" amino acids 42 to 70 (29 residues), see Phobius details amino acids 90 to 114 (25 residues), see Phobius details amino acids 126 to 148 (23 residues), see Phobius details amino acids 155 to 181 (27 residues), see Phobius details amino acids 193 to 215 (23 residues), see Phobius details amino acids 246 to 267 (22 residues), see Phobius details amino acids 279 to 298 (20 residues), see Phobius details amino acids 305 to 324 (20 residues), see Phobius details amino acids 337 to 357 (21 residues), see Phobius details amino acids 363 to 386 (24 residues), see Phobius details PF00033: Cytochrome_B" amino acids 33 to 218 (186 residues), 254 bits, see alignment E=1.4e-79 PF13631: Cytochrom_B_N_2" amino acids 102 to 270 (169 residues), 158.8 bits, see alignment E=1.9e-50 PF00032: Cytochrom_B_C" amino acids 275 to 376 (102 residues), 147.2 bits, see alignment E=2.6e-47

Best Hits

Swiss-Prot: 69% identical to CYB_RHORU: Cytochrome b (petB) from Rhodospirillum rubrum

KEGG orthology group: K00412, ubiquinol-cytochrome c reductase cytochrome b subunit [EC: 1.10.2.2] (inferred from 89% identity to azl:AZL_c04840)

MetaCyc: 59% identical to cytochrome bc1 complex cytochrome b subunit (Arabidopsis thaliana col)

Predicted SEED Role

"Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.10.2.2

Use Curated BLAST to search for 1.10.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>AZOBR_RS20750 cytochrome B (Azospirillum brasilense Sp245)
MAQAHAPQFKNPVVKWIDSRLPIFTMLDHEYNQYPMPRNVNYLWAFGAIAGIMLVIMIAT
GLVLAMQYAANTHIAFESVERIMRDVNYGWLLRYVHANGASMFFIAVYIHMFRGLYYGSY
KAPREILWILGVLILLAMMATAFMGYVLPWGQMSFWGATVITNLFSAFPLVGDPIVTWLW
GGFSVDNPTLNRFFALHFLLPFVLLGLVVLHTAALHVSGSNNPLGIDAKGPQDTVPFNPY
VTIKDGFAVVVFLIFYAAFVFFAPNYLGHPDNYIPANPLVTPAHIVPEWYFLPFYAILRA
IPDKLGGVLAMFGAIAVLAALPWLDTSKVRSCKFRPIYRQFFWILAIDALVLGYAGAMPA
EGVWLLVARIGTAYYFFHFLILLPLLGKLERPLPLPASICEPVLKGGGRVAAGAHAKPME
KA