Protein Info for AZOBR_RS20515 in Azospirillum brasilense Sp245

Annotation: alkyl hydroperoxide reductase subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 526 TIGR03140: alkyl hydroperoxide reductase subunit F" amino acids 1 to 516 (516 residues), 804.5 bits, see alignment E=1.8e-246 PF13192: Thioredoxin_3" amino acids 124 to 194 (71 residues), 29 bits, see alignment E=3.7e-10 PF07992: Pyr_redox_2" amino acids 212 to 503 (292 residues), 167.6 bits, see alignment E=1.7e-52 PF12831: FAD_oxidored" amino acids 213 to 241 (29 residues), 28.1 bits, see alignment (E = 5.5e-10) PF13738: Pyr_redox_3" amino acids 258 to 486 (229 residues), 52.7 bits, see alignment E=1.6e-17 PF00070: Pyr_redox" amino acids 356 to 428 (73 residues), 46.9 bits, see alignment E=1.3e-15

Best Hits

Swiss-Prot: 78% identical to AHPF_PSEPK: Alkyl hydroperoxide reductase subunit F (ahpF) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03387, alkyl hydroperoxide reductase subunit F [EC: 1.6.4.-] (inferred from 80% identity to rru:Rru_A1417)

MetaCyc: 64% identical to alkyl hydroperoxide reductase, AhpF component (Escherichia coli K-12 substr. MG1655)
RXN-8506 [EC: 1.5.1.37]; R4-RXN [EC: 1.5.1.37, 1.11.1.26]

Predicted SEED Role

"Alkyl hydroperoxide reductase protein F (EC 1.6.4.-)" in subsystem Thioredoxin-disulfide reductase (EC 1.6.4.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.4.-

Use Curated BLAST to search for 1.11.1.26 or 1.5.1.37 or 1.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ANX4 at UniProt or InterPro

Protein Sequence (526 amino acids)

>AZOBR_RS20515 alkyl hydroperoxide reductase subunit F (Azospirillum brasilense Sp245)
VLDSNVKGQLKAYFDKITQPIELAASLDDGAKSQDMQALLQDIASLSDKITFVRTDDDAR
KPSFAIKRTGTDVSVRFAGLPMGHEFNSLILALLQVGGHPSKESAEVIEQVKALEGEYSF
ETYFSLSCQNCPDVVQALNLMSVLNPRIKHVAIDGALFQQEVDSRKIMAVPSIFLNGEPF
GQGRMGLEQILAKIDTGASQRDAEKIKAKDAFDVLVIGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMAIENFISVSHTEGPKLATALEQHVKEYEVDVMNLQRAEKLIPAEGPGG
LIEIQLANGASLKSKTVILSTGARWRQMGVPGEDEYRNKGVAYCPHCDGPLFKGKRVSVI
GGGNSGVEAAIDLAGIVAEVTLIEFDSQLRADEVLQRKLRSLPNVRVITSGLTTEVHGDG
TKVTGLSYKNRNTGEVHRIDLEGIFVQIGLVPNTEWLKGSVALSPRGEIEVDARGQTSIP
GVFAAGDATTVPYKQIIIAMGEGSKAALSAFDYLIRMAPVEAVAAA