Protein Info for AZOBR_RS18565 in Azospirillum brasilense Sp245

Updated annotation (from data): Nitrite reductase (NAD(P)H) (EC 1.7.1.4)
Rationale: Specifically important for utilizing Sodium nitrate. Automated validation from mutant phenotype: the predicted function (1.7.1.4) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: nitrite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 821 PF07992: Pyr_redox_2" amino acids 13 to 291 (279 residues), 192.8 bits, see alignment E=3.2e-60 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 14 to 803 (790 residues), 966.7 bits, see alignment E=5.5e-295 PF00070: Pyr_redox" amino acids 155 to 234 (80 residues), 69.4 bits, see alignment E=1.1e-22 PF18267: Rubredoxin_C" amino acids 325 to 391 (67 residues), 68.4 bits, see alignment E=1.5e-22 PF04324: Fer2_BFD" amino acids 429 to 477 (49 residues), 50.4 bits, see alignment 7.8e-17 amino acids 493 to 542 (50 residues), 44.4 bits, see alignment 5.9e-15 PF03460: NIR_SIR_ferr" amino acids 568 to 632 (65 residues), 58.6 bits, see alignment E=1.5e-19 PF01077: NIR_SIR" amino acids 641 to 776 (136 residues), 110.6 bits, see alignment E=1.8e-35

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 80% identity to azl:AZL_e03500)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8ASU2 at UniProt or InterPro

Protein Sequence (821 amino acids)

>AZOBR_RS18565 Nitrite reductase (NAD(P)H) (EC 1.7.1.4) (Azospirillum brasilense Sp245)
MSTHTNPPTPRERLVVVGNGMAGIRTLEELLAKAPDRYDITVFGAEPHPNYNRIMLSPVL
AGEKTFEQIVLNGRDWYEANGIRLLTGDRVEVIDRANCTVTAISGLTVPYDKLLIATGST
PVIIPVPGSTLTGVVGFRDLADVDTMLEAAARGGRAVVIGGGLLGLEAANGLKVKGMDVT
VLHLMDTLMERQLDRSAGALLRHELERRGITVLTGADTAEIVGSEKVSAVRLKNGQELPA
DLVVMAVGIRPNTTLGKAAGLACGRGIQVDDAMTTSDPAILSVGECVEHRGQTYGLVAPL
FEMAKVAAARLAGSAEAAYTGSVTSTKLKVTGVDVFSAGDFTGGKDCEDIVFRDAARGVY
KRVVVKENRILGAVLYGDTKDGGWYFQMLKDGTEVAPVRDTLIFGQSFGGEGAANPKAAV
AALPDSAEICGCNGVCKGTIVTAITEKGLSSLDEVRAHTKASASCGSCTGQVEQLLALTL
GDGYAGEAKAKPMCKCTDRTHDEVRRAITALDLKTIPDVMQRLEWRTPDGCHHCRPALNY
YLLCEWPGEYKDDSRSRYINERVHANIQKDGTYSVVPRMWGGLTSAKELRAIADVVDKFA
IPTVKVTGGQRIDLFGVKKEDLPAVWADLNAAGMVSGHAYAKGLRTVKTCVGSEWCRFGT
QDSTGLGVKLERMTWGTWTPHKVKLAVSGCPRNCAEATIKDLGVVCVDSGYELHVGGNGG
MHVRACDLLVKVETEAEVLEYTGAYMQLYREEARYLERTAPWVERVGLDHIRRRLVDDAE
GRAALNARFLFSQSFSQDDPWAERANGSVDRHEFAPLAQVG